Recombinant Rat PDGF-AA Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Citations (12)
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Recombinant Rat PDGF-AA Protein, CF Summary

Product Specifications

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 3-9 ng/mL.
E. coli-derived rat PDGF-AA protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.5 kDa (monomer)

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: PDGF-AA

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB,-BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that have five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. Rat PDGF-A chain cDNA encodes a 204 amino acid (aa) residue precursor protein with a 20 aa signal peptide, a 65 aa propeptide that is removed by proteolysis, and a 119 aa mature protein. By alternative splicing, a short form lacking 8 C-terminal aa residues also exists. The long form contains the 8 aa basic insert which promotes intracellular cell retention and association with cell matrix. PDGF-A is expressed in multiple cell types and tissues. Based on PDGF-A knockout studies, PDGF-A appears to be important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles and intestinal villus (1).

  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
Long Name
Platelet-derived Growth Factor AA
Entrez Gene IDs
5154 (Human); 25266 (Rat)
Alternate Names

Citations for Recombinant Rat PDGF-AA Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Temporal control of PDGFRalpha regulates the fibroblast-to-myofibroblast transition in wound healing
    Authors: L Yao, BH Rathnakar, HR Kwon, H Sakashita, JH Kim, A Rackley, JJ Tomasek, WL Berry, LE Olson
    Cell Reports, 2022-08-16;40(7):111192.
    Species: Mouse
    Sample Types: Serum
    Applications: Bioassay
  2. Tension Sensor Based on Fluorescence Resonance Energy Transfer Reveals Fiber Diameter-Dependent Mechanical Factors During Myelination
    Authors: T Shimizu, H Murakoshi, H Matsumoto, K Ichino, A Hattori, S Ueno, A Ishida, N Tajiri, H Hida
    Frontiers in Cellular Neuroscience, 2021-08-02;15(0):685044.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Genetic analyses in mouse fibroblast and melanoma cells demonstrate novel roles for PDGF-AB ligand and PDGF receptor alpha
    Authors: JL Kadrmas, MC Beckerle, M Yoshigi
    Sci Rep, 2020-11-09;10(1):19303.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Phthalate inhibits Leydig cell differentiation and promotes adipocyte differentiation
    Authors: X Hao, X Guan, X Zhao, M Ji, X Wen, P Chen, F Chen, J Yang, Q Lian, L Ye, H Chen
    Chemosphere, 2020-08-06;262(0):127855.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  5. Transplanted Oligodendrocyte Progenitor Cells Survive in the Brain of a Rat Neonatal White Matter Injury Model but Less Mature in Comparison with the Normal Brain
    Authors: S Ogawa, M Hagiwara, S Misumi, N Tajiri, T Shimizu, A Ishida, N Suzumori, M Sugiura-Og, H Hida
    Cell Transplant, 2020-01-01;29(0):9636897209460.
    Species: Rat, Transgenic Rat
    Sample Types: Whole Cells
    Applications: Bioassay, Cell Culture
  6. Characterization of stem cells associated with seminiferous tubule of adult rat testis for their potential to form Leydig cells
    Authors: X Guan, P Chen, X Zhao, X Hao, F Chen, M Ji, X Wen, H Lin, L Ye, H Chen
    Stem Cell Res, 2019-10-15;41(0):101593.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  7. Growth factor signaling regulates mechanical nociception in flies and vertebrates
    Authors: R Lopez-Bell, S Puig, PJ Huang, CR Tsai, HN Turner, MJ Galko, HB Gutstein
    J. Neurosci., 2019-05-28;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay, In Vivo
  8. Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis
    Cell Rep, 2016-11-08;17(7):1832-1844.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Autophagy modulates SNCA/alpha-synuclein release, thereby generating a hostile microenvironment.
    Authors: Poehler A, Xiang W, Spitzer P, May V, Meixner H, Rockenstein E, Chutna O, Outeiro T, Winkler J, Masliah E, Klucken J
    Autophagy, 2014-01-01;10(12):2171-92.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. BMP4 is required for the initial expression of MITF in melanocyte precursor differentiation from embryonic stem cells.
    Authors: Yang J, Wang J, Pan L, Li H, Rao C, Zhang X, Niu G, Qu J, Hou L
    Exp Cell Res, 2013-09-27;320(1):54-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium.
    Authors: Schneider L, Stock CM, Dieterich P, Jensen BH, Pedersen LB, Satir P, Schwab A, Christensen ST, Pedersen SF
    J. Cell Biol., 2009-04-06;185(1):163-76.
    Species: Hamster, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
    Authors: Yang X, Chrisman H, Weijer CJ
    Development, 2008-10-02;135(21):3521-30.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay


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Recombinant Rat PDGF-AA Protein, CF
By Anonymous on 07/05/2017
Application: Cell migration/motility