Recombinant Rat PDGF-AA Protein, CF

Catalog # Availability Size / Price Qty
1055-AA-050
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Recombinant Rat PDGF-AA Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 3-9 ng/mL.
Source
E. coli-derived rat PDGF-AA protein
Ser87-Arg196
Accession #
N-terminal Sequence
Analysis
Ser87
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.5 kDa (monomer)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1055-AA

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: PDGF-AA

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB,-BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that have five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. Rat PDGF-A chain cDNA encodes a 204 amino acid (aa) residue precursor protein with a 20 aa signal peptide, a 65 aa propeptide that is removed by proteolysis, and a 119 aa mature protein. By alternative splicing, a short form lacking 8 C-terminal aa residues also exists. The long form contains the 8 aa basic insert which promotes intracellular cell retention and association with cell matrix. PDGF-A is expressed in multiple cell types and tissues. Based on PDGF-A knockout studies, PDGF-A appears to be important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles and intestinal villus (1).

References
  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
Long Name
Platelet-derived Growth Factor AA
Entrez Gene IDs
5154 (Human); 25266 (Rat)
Alternate Names
PDGFAA; PDGF-AA

Citations for Recombinant Rat PDGF-AA Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis
    Cell Rep, 2016;17(7):1832-1844.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. BMP4 is required for the initial expression of MITF in melanocyte precursor differentiation from embryonic stem cells.
    Authors: Yang J, Wang J, Pan L, Li H, Rao C, Zhang X, Niu G, Qu J, Hou L
    Exp Cell Res, 2014;320(1):54-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Autophagy modulates SNCA/alpha-synuclein release, thereby generating a hostile microenvironment.
    Authors: Poehler A, Xiang W, Spitzer P, May V, Meixner H, Rockenstein E, Chutna O, Outeiro T, Winkler J, Masliah E, Klucken J
    Autophagy, 2014;10(12):2171-92.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium.
    Authors: Schneider L, Stock CM, Dieterich P, Jensen BH, Pedersen LB, Satir P, Schwab A, Christensen ST, Pedersen SF
    J. Cell Biol., 2009;185(1):163-76.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  5. PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
    Authors: Yang X, Chrisman H, Weijer CJ
    Development, 2008;135(21):3521-30.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Rat PDGF-AA Protein, CF

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Recombinant Rat PDGF-AA Protein, CF
By Anonymous on 07/05/2017
Application: Cell migration/motility
Reason for Rating: TRIED IT ON NIH 3T3S MOUSE FIBROBLAST TO ESTABLISH A CHEMOTACTIC GRADIENT AND IT WORKED VERY WELL. THE RESULTS WERE AS EXPECTED.