Recombinant Rat TNF RI/TNFRSF1A Fc Chimera Protein, CF

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7990-TR-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Rat TNF RI/TNFRSF1A Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.5-3 ng/mL in the presence of 0.1 ng/mL of Recombinant Rat TNF‑ alpha (Catalog # 510-RT).
Source
Mouse myeloma cell line, NS0-derived rat TNF RI/TNFRSF1A protein
Rat TNF RI/TNFRSF1A
(Leu30-Ala211)
Accession # P22934
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47.3 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions

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7990-TR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7990-TR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R‑p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Rat TNF RI is a 461 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa extracellular domain (ECD) with a PLAD (pre‑ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 227 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of rat TNF RI shares 69%, 88%, 66%, 68% and 75% aa sequence identity with human, mouse, canine, feline and porcine TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF‑ alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF‑ beta /TNFSF1B (lymphotoxin‑ alpha ) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro‑inflammatory stimuli (7). TNF‑ alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8  9, 16). Release of the 28‑34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (13). Release helps to resolve inflammatory reactions, since it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR‑associated periodic syndrome) (7).

References
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Chan, F.K. et al. (2000) Science 288:2351.
  4. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  6. Banner, D.W et al. (1993) Cell 73: 431.
  7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
  8. Legler, D.F. et al. (2003) Immunity 18:655.
  9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  11. Jin, L. et al. (2000) J. Immunol. 165:5153.
  12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  15. McDermott, M.F. et al. (1999) Cell 97:133.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

Citation for Recombinant Rat TNF RI/TNFRSF1A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Selective activation of microglia facilitates synaptic strength.
    Authors: Clark A, Gruber-Schoffnegger D, Drdla-Schutting R, Gerhold K, Malcangio M, Sandkuhler J
    J Neurosci, 2015;35(11):4552-70.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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