Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF

HEK293 Expressed
Catalog # Availability Size / Price Qty
SDS-PAGE gel of purified recombinant SARS-CoV-2 Spike S2 Domain protein with His-tag
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Product Details
Citations (4)
Reviews (1)

Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Bioassay data are not available.
Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike S2 Subunit protein
Ser686-Lys1211, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
60 kDa
80-100 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE SDS-PAGE gel of purified recombinant SARS-CoV-2 Spike S2 Domain protein with His-tag View Larger

2 μg/lane of Recombinant SARS-CoV-2 Spike S2 Subunit His-tag (Catalog # 10594-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-100 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Spike S2 Subunit

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). As with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (2-4). A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV2, similar to SARS-CoV-1, through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (5, 6). The S2 subunit of SARS-CoV-2 shares 90% and 41% amino acid sequence identity with the S2 subunit of SARS-CoV-1 and MERS, respectively. It has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (7, 8).

  1. Rota, P.A. et al. (2003) Science 300:1394.
  2. Bosch, B.J. et al. (2003). J. Virol. 77:8801.
  3. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:5871.
  4. Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
  5. Li, W. et al. (2003) Nature 426:450.
  6. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  7. Wang, X. et al. (2020)
  8. Wang, K. et al. (2020) bioRxiv
Long Name
Spike Protein, S2 Subunit
Alternate Names
COVID-19; S2 protein; SARS-CoV-2 Spike; SARS-CoV-2; Spike glycoprotein Subunit 2; Spike protein S2; spike protein; Spike S2 Subunit; structural protein; surface glycoprotein

Citations for Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Class switch towards spike protein-specific IgG4 antibodies after SARS-CoV-2 mRNA vaccination depends on prior infection history
    Authors: Kiszel, P;Sík, P;Miklós, J;Kajdácsi, E;Sinkovits, G;Cervenak, L;Prohászka, Z;
    Scientific reports
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. Evidence, detailed characterization and clinical context of complement activation in acute multisystem inflammatory syndrome in children
    Authors: G Sinkovits, J Schnur, L Hurler, P Kiszel, ZZ Prohászka, P Sík, E Kajdácsi, L Cervenak, V Maráczi, M Dávid, B Zsigmond, É Rimanóczy, C Bereczki, L Willems, EJM Toonen, Z Prohászka
    Scientific Reports, 2022-11-17;12(1):19759.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Standard
  3. Children develop robust and sustained cross-reactive spike-specific immune responses to SARS-CoV-2 infection
    Authors: AC Dowell, MS Butler, E Jinks, G Tut, T Lancaster, P Sylla, J Begum, R Bruton, H Pearce, K Verma, N Logan, G Tyson, E Spalkova, S Margielews, GS Taylor, E Syrimi, F Baawuah, J Beckmann, IO Okike, S Ahmad, J Garstang, AJ Brent, B Brent, G Ireland, F Aiano, Z Amin-Chowd, S Jones, R Borrow, E Linley, J Wright, R Azad, D Waiblinger, C Davis, EC Thomson, M Palmarini, BJ Willett, WS Barclay, J Poh, G Amirthalin, KE Brown, ME Ramsay, J Zuo, P Moss, S Ladhani
    Nature Immunology, 2021-12-22;23(1):40-49.
    Species: Human
    Sample Types: Plasma
    Applications: Neutralization
  4. Humoral immune responses during SARS-CoV-2 mRNA vaccine administration in seropositive and seronegative individuals
    Authors: E Fraley, C LeMaster, E Geanes, D Banerjee, S Khanal, E Grundberg, R Selvaranga, T Bradley
    Bmc Medicine, 2021-07-26;19(1):169.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay


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Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF
By Anonymous on 08/06/2023
Application: In vitro bioactivity in cell culture
Reason for Rating: Worked great for in vitro testing against THP-1 cells.