Reelin Antibody (G10) - BSA Free

Novus Biologicals | Catalog # NB600-1081

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # G10

Format

BSA Free
View all Reelin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein, corresponding to amino acids 164-496 of Mouse Reelin (derived from E15-E17 embryonic brain).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Secreted

Specificity

Reelin (G10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

388 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Reelin Antibody (G10) - BSA Free

Immunohistochemistry-Frozen: Reelin Antibody (G10) - BSA Free [NB600-1081]

Immunohistochemistry-Frozen: Reelin Antibody (G10) - BSA Free [NB600-1081]

Immunohistochemistry-Frozen: Reelin Antibody (G10) [NB600-1081] - Frozen mouse brain coronal section stained with Reelin Antibody (G10). IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: Reelin Antibody (G10) - BSA Free [NB600-1081]

Immunohistochemistry: Reelin Antibody (G10) - BSA Free [NB600-1081]

Immunohistochemistry: Reelin Antibody (G10) [NB600-1081] - Mouse anti-Reelin staining of embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone, positive for reelin using NB600-1081.
Immunohistochemistry: Reelin Antibody (G10) - BSA Free [NB600-1081]

Immunohistochemistry: Reelin Antibody (G10) - BSA Free [NB600-1081]

Immunohistochemistry: Reelin Antibody (G10) [NB600-1081] - Mouse anti-Reelin. Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS. The blue stain is Hoechst nuclear counter stain. Photo courtesy of Dr. Jason Emsley, Harvard Medical School, Boston, MA.

Applications for Reelin Antibody (G10) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 27915032).

Reviewed Applications

Read 1 review rated 5 using NB600-1081 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

20mM Phosphate and 0.25M NaCl

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Reelin

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Alternate Names

Reeler, RELN, RL

Entrez Gene IDs

5649 (Human); 19699 (Mouse); 24718 (Rat)

Gene Symbol

RELN

UniProt

P78509

Additional Reelin Products

Product Documents for Reelin Antibody (G10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Reelin Antibody (G10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Reelin Antibody (G10) - BSA Free

Customer Reviews for Reelin Antibody (G10) - BSA Free (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • Reelin Antibody (G10)
    Name: Bhavin Shah
    Application: Immunofluorescence in frozen sections
    Sample Tested: 20 um fixed frozen mouse brain coronal section
    Species: Mouse
    Verified Customer | Posted 11/25/2021
    Anti-Reelin antibody review. Excellent monooclonal antibody to determine Reelin positive cells.
    Antigen Retrievel: Using 10mM sodium citrate and boiling in a microwave for 2 min. The antibody works without the antigen retrieval also.
    Reelin Antibody (G10) - BSA Free NB600-1081

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Protocols

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FAQs

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