RelA/NFkB p65 Antibody Pack

Name: RelA/NFkB p65 Antibody Pack
Brand: Novus Biologicals
SKU: NBP3-20216
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-20216

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Product Summary for RelA/NFkB p65 Antibody Pack

This pack contains 1 vial each of: MAB7226-SP (25 ug), MAB7226-SP (0.025 mg), NB100-56712SS (25 ug), MAB7624-SP (1 mL).

Product Specifications

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for RelA/NFkB p65 Antibody Pack

Detection of Phospho-RelA/NF kappa B p65 (S536) in Treated HT-29 and Treated HeLa Cell Lines by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB7226) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-RelA/ NF kappa B p65 (S536) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of RelA/NFkB p65 in Ramos, Daudi and NIH-3T3 Cell Lines by Western Blot.

Analysis of P65 using p65 antibody at 2 ug/mL in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.Western blot shows 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell line lysates. RelA/NFkB p65 was detected with 2 µg/mL of Mouse Anti-Human RelA/NFkB p65 (112A1021) Monoclonal Antibody (Catalog # NB100-56712) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. This experiment was conducted under reducing conditions.

Detection of Phospho-RelA/NF kappa B p65 (S529) in Treated HeLa by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S529) Monoclonal Antibody (Catalog # MAB7624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-RelA/NF kappa B p65 (S529) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Kit Contents for RelA/NFkB p65 Antibody Pack

MAB7226-SP
MAB7624-SP
HAF007: Mouse IgG Horseradish Peroxidase-conjugated Antibody
NB100-56712SS

Formulation, Preparation, and Storage

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Storage

Storage is content dependent.

Background: RelA/NFkB p65

Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes. The family members include p50, p52, p65 (RelA), c-Rel, and RelB. The Nf-kB p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein. Association with inhibitory proteins of the I B family, retains NF- B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA. In addition to the nuclear translocation and DNA binding, Nf-kB's transcriptional activity is also regulated by coactivators and corepressors in the nucleus. Interaction of p65 with coactivators; CREB-binding protein and p300, increases its transcriptional activity.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Gene Symbol

RELA

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Product Documents for RelA/NFkB p65 Antibody Pack

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Product Specific Notices for RelA/NFkB p65 Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.