RelA/NFkB p65 [p Ser536] Antibody
Novus Biologicals | Catalog # NB100-82088
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Fish, Monkey
Cited:
Human, Mouse, Rat, Fish
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human RelA/NFkB p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).
Reactivity Notes
Use in Labeo bata reported in secitific publication PMID: 32800225
Modification
p Ser536
Specificity
Detects endogenous levels of RelA/NFkB p65 only when phosphorylated at serine 536.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RelA/NFkB p65 [p Ser536] Antibody
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088]
Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Methanol-fixed MEF cellsImmunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088]
Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Paraffin-embedded human breast carcinoma tissue.Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088]
Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Paraffin-embedded human Lung carcinoma tissueWestern Blot: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] -
Western Blot: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Western Blot analysis of various cells using NB100-82088 diluted at 1:2000Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] -
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-82088.Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] -
Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-82088 incubated with blocking peptide (right)Western Blot: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] -
Effects of RHSL on the translocation of phosphorylated NF-kappa B into the nucleus in TLR-7 agonist-stimulated RAW264.7 cells. Cells were treated with a concentration of 10 μg/mL TLR-7 agonist or 100 μg/mL RHSL, either alone or in combination, for 24 h. Nuclear-cytoplasmic fractionation analysis was performed to quantify the levels of phosphorylated NF-kappa B and total NF-kappa B in cytoplasmic (A) and nuclear (B) protein extracts, respectively. GAPDH and Lamin A/C were used as internal controls for cytoplasmic and nuclear fractions, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39337618), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] -
Effects of RHSL on the translocation of phosphorylated NF-kappa B into the nucleus in TLR-7 agonist-stimulated RAW264.7 cells. Cells were treated with a concentration of 10 μg/mL TLR-7 agonist or 100 μg/mL RHSL, either alone or in combination, for 24 h. Nuclear-cytoplasmic fractionation analysis was performed to quantify the levels of phosphorylated NF-kappa B and total NF-kappa B in cytoplasmic (A) and nuclear (B) protein extracts, respectively. GAPDH and Lamin A/C were used as internal controls for cytoplasmic and nuclear fractions, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39337618), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for RelA/NFkB p65 [p Ser536] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-200
Immunohistochemistry
1:100-1:300
Immunohistochemistry-Paraffin
1:50-1:100
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 50% glycerol, 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RelA/NFkB p65
Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Alternate Names
NFkB p65, NFKB3, p65RelA, NF kB p65 phospho, NFkB p65 phospho, NF-kB p65 phospho, phospho NF kB p65, phospho NFkB p65, phospho NF-kB p65
Gene Symbol
RELA
UniProt
Additional RelA/NFkB p65 Products
Product Documents for RelA/NFkB p65 [p Ser536] Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RelA/NFkB p65 [p Ser536] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for RelA/NFkB p65 [p Ser536] Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars