Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25628
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Lateral Flow Assay
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1248 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of Respiratory Syncytial Virus Nucleoprotein. The exact sequence is proprietary.
Reactivity Notes
Respiratory Syncytial Virus
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) were used to detect the bound primary antibodies.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (NBP3-25626) conjugated with HRP (1 ug/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (NBP3-25626) conjugated with HRP (1 ug/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 ug/mL) below. Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) 3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (NBP3-25626)
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (NBP3-25626) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (NBP3-25626) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
Immunocytochemistry/Immunofluorescence: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
Immunocytochemistry/Immunofluorescence: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Respiratory Syncytial virus Nucleoprotein antibody [HL1248] detects Respiratory Syncytial virus Nucleoprotein protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Respiratory Syncytial virus Nucleoprotein stained by Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) diluted at 1:500. Blue: Fluoroshield with DAPI.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) were used to detect the bound primary antibodies.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) conjugated with HRP (1 ug/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (NBP3-25626) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) conjugated with HRP (1 ug/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus nucleoprotein using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody (NBP3-25628) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) conjugated with HRP (1 ug/mL) Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (NBP3-25626) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
Lateral Flow Assay: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
Lateral Flow Assay: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Detection of respiratory syncytial virus type A nucleoprotein (0.5 ug/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Indirect ELISA analysis performed by coating plate with recombinant E.coli expressed, C-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10^-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) 3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (NBP3-25626)
Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Non-infected (-) and infected (+) HEp-2 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (NBP3-25628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lateral Flow Assay: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] -
Lateral Flow Assay: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) - Azide and BSA Free [NBP3-25628] - Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair. Capture: Respiratory Syncytial virus Nucleoprotein antibody (NBP3-25628) [HL1248]) Detection: Respiratory Syncytial virus Nucleoprotein antibody (NBP3-25626) [HL1246]) Samples (25 ng) : 1. RSV type A Nucleoprotein 2. RSV type B Nucleoprotein 3. SARS-CoV-2 Nucleocapsid protein 4. FluA (H1N1) Nucleoprotein 5. FluA (H3N2) Nucleoprotein 6. FluB Nucleoprotein 7. Lysis buffer
Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1248) [NBP3-25628] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial Virus Nucleoprotein antibody [HL1248] (NBP3-25628) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications
Application
Recommended Usage
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Respiratory Syncytial Virus Nucleoprotein
Additional Respiratory Syncytial Virus Nucleoprotein Products
Product Documents
Certificate of Analysis
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Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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