Rev-erb A alpha/NR1D1 Antibody

Novus Biologicals | Catalog # NBP2-19574

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (91%), Rat (93%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Rev-erb A alpha/NR1D1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rev-erb A alpha/NR1D1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rev-erb A alpha/NR1D1 Antibody

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] - NR1D1 antibody detects NR1D1 protein at cytosol and nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NR1D1 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574]

Immunohistochemistry-Paraffin: NR1D1 Antibody [NBP2-19574] - NR1D1 antibody detects NR1D1 protein at nucleus on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NR1D1 antibody dilution: 1:500.
Rev-erb A alpha/NR1D1 Antibody

Western Blot: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NR1D1 antibody (NBP2-19574) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rev-erb A alpha/NR1D1 Antibody

Western Blot: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NR1D1 antibody (NBP2-19574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Rev-erb A alpha/NR1D1 Antibody

Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] -

NR1D1 antibody detects NR1D1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NR1D1 protein stained by NR1D1 antibody (NBP2-19574) diluted at 1:400.
Blue: Hoechst 33342 staining.

Applications for Rev-erb A alpha/NR1D1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rev-erb A alpha/NR1D1

Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.

Alternate Names

Ear-1, NR1D1, Reverb A alpha, THRA1, THRAL

Gene Symbol

NR1D1

UniProt

P20393 (Human)

Additional Rev-erb A alpha/NR1D1 Products

Product Documents for Rev-erb A alpha/NR1D1 Antibody

Certificate of Analysis

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Product Specific Notices for Rev-erb A alpha/NR1D1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Rev-erb A alpha/NR1D1 Antibody

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Protocols

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