ReZolve-ER™

Catalog # Availability Size / Price Qty
6640/1800U
Cat.No. 6640 - ReZolve-ER | C21H12N7O3Re | CAS No. 1404104-40-0
1 Image
Description: Fluorescent ER and plasma membrane stain

Chemical Name: (OC-6-33)-Tricarbonyl(1,10-phenanthroline-κN1N10)[4-(2H-terazol-5-yl-κN2)pyridinato]Rhenium

Purity: ≥95%

Product Details
Reviews

Biological Activity

Fluorescent endoplasmic reticulum and plasma membrane stain. Exhibits rapid uptake in live cells. Displays low cytotoxicity and is resistant to photobleaching. Suitable for imaging live and fixed cells in short and long time course experiments. Compatible with fluorescent microscopy using single photon excitation at 403 nm or two-photon excitation between 800 and 830 nm. Excitation/emission λ: 350-405 nm / 500-650 nm.

PNT2 prostate epithelial cell line stained with ReZolve-ER™  PNT2 prostate epithelial cell line stained with ReZolve-ER™ (green) and CellMask (purple)*

Application of ReZolve-ER™ on PNT2 prostate epithelial cells; Left: PNT2 prostate epithelial cells stained with ReZolve-ER™. Right: PNT2 prostate epithelial cells stained with ReZolve-ER™ (green) and CellMask (purple)*.

Cells are incubated with growth media containing 20 μM ReZolve-ER™ for 10-20 min before imaging. Cells were viewed using a Nikon+A1 confocal microscope, ReZolve-ER™ is excited at 403 nm and emission is collected using a 525 nm GaaS PMT detect.

*CellMask was purchased from Thermo Fisher

Technical Data

M.Wt:
596.58
Formula:
C21H12N7O3Re
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
1404104-40-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time.
    Bader et al.
    FEBS Lett., 2016;590:3051
  2. Bright lights down under: Metal ion complexes turning the spotlight on metabolic processes at the cellular level.
    Gillam et al.
    Coordination Chem.Rev., 2018;375:234

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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