ReZolve-ER™

Biological Activity

Fluorescent endoplasmic reticulum and plasma membrane stain. Exhibits rapid uptake in live cells. Displays low cytotoxicity and is resistant to photobleaching. Suitable for imaging live and fixed cells in short and long time course experiments. Compatible with fluorescent microscopy using single photon excitation at 403 nm or two-photon excitation between 800 and 830 nm. Excitation/emission λ: 350-405 nm / 500-650 nm.

Application of ReZolve-ER™ on PNT2 prostate epithelial cells

Left: PNT2 prostate epithelial cells stained with ReZolve-ER™. Right: PNT2 prostate epithelial cells stained with ReZolve-ER™ (green) and CellMask (purple)*.

Cells are incubated with growth media containing 20 μM ReZolve-ER™ for 10-20 min before imaging. Cells were viewed using a Nikon+A1 confocal microscope, ReZolve-ER™ is excited at 403 nm and emission is collected using a 525 nm GaaS PMT detect.

_{*CellMask was purchased from Thermo Fisher}

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time.
   Bader et al.
   FEBS Lett., 2016;590:3051
2. Bright lights down under: Metal ion complexes turning the spotlight on metabolic processes at the cellular level.
   Gillam et al.
   Coordination Chem.Rev., 2018;375:234

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