RGS16 Antibody - BSA Free

Novus Biologicals | Catalog # NB300-350

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Chicken

Format

BSA Free
View all RGS16 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: corresponding to amino acids 130-202 of Human RGS16 (NP_002919.1).

Reactivity Notes

Cross-reacts with Human, Mouse and Rat..Not yet tested in other species.

Specificity

RGS16

Clonality

Polyclonal

Host

Chicken

Scientific Data Images for RGS16 Antibody - BSA Free

Western Blot: RGS16 AntibodyBSA Free [NB300-350]

Western Blot: RGS16 AntibodyBSA Free [NB300-350]

Western Blot: RGS16 Antibody [NB300-350] - E coli-derived fusion protein as test antigen. Affi-anti- RGS16 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-RGS16 )

Applications for RGS16 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:200-1:500
Application Notes
ICC: Use at a dilution of 1/200. WB: Use at a dilution of 1/2000. Predicted molecular weight: 24 kDa. Optimal dilutions/concentrations should be determined by the end user. Use in Immunohistochemistry reported in scientific literature (PMID: 23858031) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23858031)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RGS16

RGS16 (regulator of G protein signalling 16) negatively regulates G protein coupled receptor signalling. RGS16 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP bound form. Binds to G(i) alpha and G(o) alpha, but not to G(s) alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.

Alternate Names

A28-RGS14, A28-RGS14P, hRGS-r, regulator of G-protein signaling 16, regulator of G-protein signalling 16, Retinally abundant regulator of G-protein signaling, Retinal-specific RGS, RGSR, RGS-R

Entrez Gene IDs

6004 (Human); 19734 (Mouse); 360857 (Rat)

Gene Symbol

RGS16

UniProt

O15492 (Human)

Additional RGS16 Products

Product Documents for RGS16 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RGS16 Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RGS16 Antibody - BSA Free

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Protocols

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FAQs

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