Rhodamine Antibody (11H10) - BSA Free
Novus Biologicals | Catalog # NBP1-69961
![Rhodamine Antibody (11H10) [NBP1-69961]](https://resources.rndsystems.com/images/products/Rhodamine-Antibody-11H10-N-A-NBP1-69961-img0002.jpg "Rhodamine Antibody (11H10) [NBP1-69961]")
![Western Blot: Rhodamine Antibody (11H10) [NBP1-69961]](https://resources.rndsystems.com/images/products/Rhodamine-Antibody-11H10-Western-Blot-NBP1-69961-img0003.jpg "Western Blot: Rhodamine Antibody (11H10) [NBP1-69961]")
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Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Western Blot, ELISA, Dot Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 11H10
Format
BSA Free
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Product Specifications
Immunogen
Rhodamine Antibody (11H10) was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.
Specificity
RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Rhodamine Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Rhodamine Antibody (11H10) - BSA Free
Western Blot: Rhodamine Antibody (11H10) [NBP1-69961]
Western Blot: Rhodamine Antibody (11H10) [NBP1-69961] - Dot Blot of Mouse anti-Rhodamine Monoclonal Antibody. Antigen: Row 1 - Rhodamine Conjugated Streptavidin Row 2 - Streptavidin. Load: Column 1 - 100 ng Column 2 - 33.3 ng Column 3 - 11.1 ng Column 4 - 3.70 ng Column 5 - 1.23 ng.Primary antibody: Mouse anti-Rhodamine Monoclonal Antibody at 1:1,000 for 60 min at RT.Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT.
Rhodamine Antibody (11H10)
Dot Blot of Mouse anti-Rhodamine Monoclonal Antibody. Antigen: Row 1 - Rhodamine Conjugated Streptavidin. Row 2 - Streptavidin. Load: Column 1 - 100ng. Column 2 - 33.3ng. Column 3 - 11.1ng. Column 4 - 3.70ng. Column 5 - 1.23ng. Primary antibody: Mouse anti-Rhodamine at 1:1,000 for 60 min at RT. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block for 1 HR at RT.Applications for Rhodamine Antibody (11H10) - BSA Free
Application
Recommended Usage
ELISA
1:10000-1:30000
Immunohistochemistry
1:1000-1:10000
Western Blot
1:1000-1:10000
Application Notes
This protein A purified monoclonal antibody against rhodamine reacts with most derivative molecules and has been tested for use in ELISA. Optimal concentration in other immunoassays should be determined by the researcher.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Rhodamine
Alternate Names
Rhodamine 6G, Rhodamine B
Additional Rhodamine Products
Product Documents for Rhodamine Antibody (11H10) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rhodamine Antibody (11H10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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