RhoG Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15747

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all RhoG Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RHOG (NP_001656.2). TVNLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCPDVPILLVGTKKDLRAQPDTLRRLKEQGQAPITPQQGQALAKQIH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RhoG Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunocytochemistry/ Immunofluorescence: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunocytochemistry/Immunofluorescence: RhoG Antibody [NBP3-15747] - Immunofluorescence analysis of L929 cells using RhoG Rabbit pAb (NBP3-15747) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunohistochemistry-Paraffin: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human appendix using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunohistochemistry-Paraffin: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human colon carcinoma using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunohistochemistry-Paraffin: RhoG Antibody - Azide and BSA Free [NBP3-15747]

Immunohistochemistry-Paraffin: RhoG Antibody [NBP3-15747] - Immunohistochemistry of paraffin-embedded human liver cancer using RhoG antibody (NBP3-15747) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
RhoG Antibody - Azide and BSA Free

Western Blot: RhoG Antibody - Azide and BSA Free [RhoG] -

Western Blot: RhoG Antibody - Azide and BSA Free [RhoG] - Western blot analysis of various lysates using RhoG Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for RhoG Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoG

RhoG, also known as Rho-related GTP-binding protein RhoG, is a 21kDa 191 amino acid protein. RhoG plays a significant part in cell migration. RhoG has been researched in relation to Tangier disease, intrahepatic cholangiocarcinoma, shigellosis, cholangiocarcinoma, cholesterol and thyroiditis. RhoG has been linked to the axon guidance, cell adhesion, phagocytosis, cell motility and cell growth pathways where it interacts with VAV1, VAV2, ELMO1, MAP3K11 and ARHGDIG.

Alternate Names

ARHGMGC125835, MGC125836, ras homolog gene family, member G (rho G), RhoG, rho-related GTP-binding protein RhoG

Gene Symbol

RHOG

Additional RhoG Products

Product Documents for RhoG Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RhoG Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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