Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG Clone # RB39659
View all RHOXF2B Primary Antibodies »

Product Specifications

Immunogen

This RHOXF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the Central region of human RHOXF2B.(Accession#:NP_001093155.1)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RHOXF2B Antibody (RB39659)

RHOXF2B Antibody (RB39659)

Western Blot:RHOXF2B Antibody (RB39659)[NBP3-52257]-

Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?).Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with De) (10 �g/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.
RHOXF2B Antibody (RB39659)

Immunocytochemistry/ Immunofluorescence:RHOXF2B Antibody (RB39659)[NBP3-52257]-

Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?).Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with De) (10 �g/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.

Applications for RHOXF2B Antibody (RB39659)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RHOXF2B

Gene Symbol

RHOXF2B

Additional RHOXF2B Products

Product Documents for RHOXF2B Antibody (RB39659)

Certificate of Analysis

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Product Specific Notices for RHOXF2B Antibody (RB39659)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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