Ribonuclease Inhibitor Antibody (OTI1B7)

Novus Biologicals | Catalog # NBP2-03254

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B7
View all Ribonuclease Inhibitor Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ribonuclease Inhibitor Antibody (OTI1B7)

Western Blot: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribonuclease Inhibitor monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunocytochemistry/ Immunofluorescence: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunocytochemistry/Immunofluorescence: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ribonuclease Inhibitor.
Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.
Flow Cytometry: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Flow Cytometry: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Flow Cytometry: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Ribonuclease Inhibitor antibody, and then analyzed by flow cytometry.
Western Blot: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Western Blot: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Western Blot: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribonuclease Inhibitor (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribonuclease Inhibitor.
Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Human lymph node tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (OTI1B7) [NBP2-03254]

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

Applications for Ribonuclease Inhibitor Antibody (OTI1B7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.66 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ribonuclease Inhibitor

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM].

Alternate Names

Placental ribonuclease inhibitor, Placental RNase inhibitor, PRI, RAIMGC4569, ribonuclease inhibitor, ribonuclease/angiogenin inhibitor, ribonuclease/angiogenin inhibitor 1MGC18200, RNHMGC54054

Entrez Gene IDs

6050 (Human)

Gene Symbol

RNH1

Additional Ribonuclease Inhibitor Products

Product Documents for Ribonuclease Inhibitor Antibody (OTI1B7)

Certificate of Analysis

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Product Specific Notices for Ribonuclease Inhibitor Antibody (OTI1B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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