RIC8A Antibody (OTI3G3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73908

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G3

Format

Azide and BSA Free
View all RIC8A Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIC8A Antibody (OTI3G3) - Azide and BSA Free

Western Blot: RIC8A Antibody (OTI3G3)Azide and BSA Free [NBP2-73908]

Western Blot: RIC8A Antibody (OTI3G3)Azide and BSA Free [NBP2-73908]

Western Blot: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8.
Immunocytochemistry/ Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908]

Immunocytochemistry/ Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908]

Immunocytochemistry/Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8.
Immunohistochemistry: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908]

Immunohistochemistry: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908]

Immunohistochemistry: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - Staining of paraffin-embedded Kidney tissue using anti-RIC8 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908]

Immunocytochemistry/ Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908]

Immunocytochemistry/Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - Staining of HepG2 cells using anti-RIC8 mouse monoclonal antibody.

Applications for RIC8A Antibody (OTI3G3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RIC8A

RIC8 is a likely orthalog of mouse Synembryn. Synembryns are proteins that positively regulate synaptic transmission. They are actively required to maintain proper activity of the Go to Gq G-protein signalling network, which regulates neurotransmitter secretion in Caenorhabditis elegans by controlling the production and consumption of diacylglycerol. In addition to its role in the adult nervous system, synembryn is required to regulate a subset of centrosome movements in the early C. elegans embryo.

Alternate Names

RIC8, RIC8A resistance to inhibitors of cholinesterase 8 homolog A (C. elegans), synembryn, synembryn-A

Gene Symbol

RIC8A

Additional RIC8A Products

Product Documents for RIC8A Antibody (OTI3G3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RIC8A Antibody (OTI3G3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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