RIG-I Antibody (4G1B6) - BSA Free

Novus Biologicals | Catalog # NBP2-61849

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 4G1B6

Format

BSA Free
View all RIG-I Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RIG-I (AA: 789-925) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

106.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIG-I Antibody (4G1B6) - BSA Free

Western Blot: RIG-I Antibody (4G1B6)BSA Free [NBP2-61849]

Western Blot: RIG-I Antibody (4G1B6)BSA Free [NBP2-61849]

Western Blot: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis using DDX58 mAb against human DDX58 (AA: 789-925) recombinant protein. (Expected MW is 42 kDa)
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

Immunocytochemistry/Immunofluorescence: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis of MCF-7 cells using DDX58 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

Flow Cytometry: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

Flow Cytometry: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis of Hela cells using DDX58 mouse mAb (green) and negative control (red).
Western Blot: RIG-I Antibody (4G1B6)BSA Free [NBP2-61849]

Western Blot: RIG-I Antibody (4G1B6)BSA Free [NBP2-61849]

Western Blot: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis using DDX58 mAb against HEK293 (1) and DDX58 (AA: 789-925)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

Immunocytochemistry/Immunofluorescence: RIG-I Antibody (4G1B6) [NBP2-61849] - Analysis of Hela cells using DDX58 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

ELISA: RIG-I Antibody (4G1B6) - BSA Free [NBP2-61849]

ELISA: RIG-I Antibody (4G1B6) [NBP2-61849] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for RIG-I Antibody (4G1B6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIG-I

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.;

Long Name

RIG-I

Alternate Names

DDX58, RIGI

Gene Symbol

RIGI

Additional RIG-I Products

Product Documents for RIG-I Antibody (4G1B6) - BSA Free

Certificate of Analysis

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Product Specific Notices for RIG-I Antibody (4G1B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RIG-I Antibody (4G1B6) - BSA Free

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Protocols

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FAQs

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