Ring finger protein 138 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86987

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ring finger protein 138 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YCPVCREVLKTPVRTTACQHVFCRKCFLTAMRESGAHCPLCRGNVTRRERACPERALDLENIMRKFSGSCRCCAKQIKFYR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ring finger protein 138 Antibody - BSA Free

Ring finger protein 138 Antibody - BSA Free Immunohistochemistry-Paraffin: Ring finger protein 138 Antibody - BSA Free [NBP1-86987]

Immunohistochemistry-Paraffin: Ring finger protein 138 Antibody - BSA Free [NBP1-86987]

Staining of human cell line A-431 shows localization to mitochondria.
Ring finger protein 138 Antibody - BSA Free Western Blot: Ring finger protein 138 Antibody - BSA Free [NBP1-86987]

Western Blot: Ring finger protein 138 Antibody - BSA Free [NBP1-86987]

Analysis in control (vector only transfected HEK293T lysate) and RNF138 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for Ring finger protein 138 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ring finger protein 138

Ring finger protein 138 is 245 amino acids long, weighs approximately 28 kDa, and has a shorter isoform that is 151 amino acids long and weighs approximately 17 kDa. Ring finger protein 138 functions as E3 ubiquitin-protein ligases that works with NLK to ubiquitinate and degrade TCF and LEF. Current research is being done on diseases and disorders related to this protein including Crohn's disease and ataxia. Ring finger protein 138 has also been shown to have interactions with PCBP4, QKI, TAF9, C6orf165, and UBE2D2 in pathways such as the antigen processing, Wnt/beta-catenin-mediated signaling, and immune system pathways.

Alternate Names

E3 ubiquitin-protein ligase RNF138, EC 6.3.2.-, hNARF, HSD-4, MGC8758, NARF, Nemo-like kinase-associated RING finger protein, ring finger protein 138NLK-associated RING finger protein, STRIN

Gene Symbol

RNF138

Additional Ring finger protein 138 Products

Product Documents for Ring finger protein 138 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Ring finger protein 138 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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