RING1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80830

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Predicted:

Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RING1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGPDGCGGEGGGAGGGDGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSLTLELVNEKFWKVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RING1 Antibody - BSA Free

Western Blot: RING1 Antibody [NBP1-80830]

Western Blot: RING1 Antibody [NBP1-80830]

Western Blot: RING1 Antibody [NBP1-80830] - Analysis in control (vector only transfected HEK293T lysate) and RING1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830]

Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Endometrium shows strong nuclear positivity in glandular cells and cells in endometrial stroma.
RING1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: RING1 Antibody [NBP1-80830]

Immunocytochemistry/ Immunofluorescence: RING1 Antibody [NBP1-80830]

Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Applications for RING1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RING1

RING1 belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq]

Long Name

E3 ubiquitin-protein ligase RING1

Alternate Names

RNF1

Entrez Gene IDs

6015 (Human)

Gene Symbol

RING1

UniProt

Q06587 (Human)

Additional RING1 Products

Product Documents for RING1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RING1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RING1 Antibody - BSA Free

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Protocols

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FAQs

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