RIOK3 Antibody (4A12-1D2) - Azide and BSA Free

Novus Biologicals | Catalog # H00008780-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4A12-1D2

Format

Azide and BSA Free
View all RIOK3 Primary Antibodies »

Product Specifications

Immunogen

RIOK3 (AAH39729, 1 a.a. ~ 516 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

Specificity

RIOK3 - RIO kinase 3 (yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RIOK3 Antibody (4A12-1D2) - Azide and BSA Free

Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Analysis of RIOK3 expression in MCF-7.
Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Western Blot analysis of RIOK3 expression in Hela NE ( Cat # L013V3 ).
Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2.Lane 1: RIOK3 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Immunoprecipitation: RIOK3 Antibody (4A12-1D2) [H00008780-M01]

Immunoprecipitation: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Analysis of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Detection limit for recombinant GST tagged RIOK3 is approximately 0.3ng/ml as a capture antibody.

Applications for RIOK3 Antibody (4A12-1D2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RIOK3

This gene was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The specific function of this gene has not yet been determined.

Alternate Names

DKFZp779L1370, EC 2.7.11.1, homolog of the Aspergillus nidulans sudD gene product, RIO kinase 3, RIO kinase 3 (yeast), serine/threonine-protein kinase RIO3, sudD (suppressor of bimD6, Aspergillus nidulans) homolog, sudD homolog, sudD suppressor of bimD6 homolog, SUDDsudD suppressor of Aspergillus nidulans bimD6 homolog

Entrez Gene IDs

8780 (Human)

Gene Symbol

RIOK3

OMIM

603579 (Human)

UniProt

AAH39729 (Human)

Additional RIOK3 Products

Product Documents for RIOK3 Antibody (4A12-1D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RIOK3 Antibody (4A12-1D2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RIOK3 Antibody (4A12-1D2) - Azide and BSA Free

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Protocols

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FAQs

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