RIPK2 Antibody (6F7) - Azide and BSA Free

Novus Biologicals | Catalog # H00008767-M02

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6F7

Format

Azide and BSA Free
View all RIPK2/RIP2 Primary Antibodies »

Product Specifications

Immunogen

RIPK2 (AAH04553, 431 a.a. ~ 540 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

RIPK2 (6F7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RIPK2 Antibody (6F7) - Azide and BSA Free

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - RIPK2 monoclonal antibody (M02), clone 6F7. Analysis of RIPK2 expression in NIH/3T3.
Immunohistochemistry-Paraffin: RIPK2 Antibody (6F7) [H00008767-M02]

Immunohistochemistry-Paraffin: RIPK2 Antibody (6F7) [H00008767-M02]

Immunohistochemistry-Paraffin: RIPK2 Antibody (6F7) [H00008767-M02] - Analysis of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 1.2 ug/ml.
Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - RIPK2 monoclonal antibody (M02), clone 6F7 Analysis of RIPK2 expression in HeLa.
Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - RIPK2 monoclonal antibody (M02), clone 6F7. Analysis of RIPK2 expression in PC-12.
Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - RIPK2 monoclonal antibody (M02), clone 6F7. Analysis of RIPK2 expression in Raw 264.7.
Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02]

Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - Analysis of RIPK2 expression in transfected 293T cell line by RIPK2 monoclonal antibody (M02), clone 6F7.Lane 1: RIPK2 transfected lysate(61.2 KDa).Lane 2: Non-transfected lysate.
ELISA: RIPK2 Antibody (6F7) [H00008767-M02]

ELISA: RIPK2 Antibody (6F7) [H00008767-M02]

ELISA: RIPK2 Antibody (6F7) [H00008767-M02] - Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.

Applications for RIPK2 Antibody (6F7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RIPK2/RIP2

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.

Long Name

Receptor Interacting Serine/Threonine Kinase 2

Alternate Names

CARD3, GIG30, RICK, RIP-2, RIP2

Entrez Gene IDs

8767 (Human)

Gene Symbol

RIPK2

OMIM

603455 (Human)

UniProt

AAH04553 (Human)

Additional RIPK2/RIP2 Products

Product Documents for RIPK2 Antibody (6F7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RIPK2 Antibody (6F7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RIPK2 Antibody (6F7) - Azide and BSA Free

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Protocols

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FAQs

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