RIZ1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89644

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RIZ1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPPFQYHHRNPMGIGVTATNFTTHNIPQTFTTAIRCTKCGKGVDNMPELHKHILACASASDKKRYTPKKNPVPLKQTVQPKNGVVVLDNSGKNAFRRMGQPKRLNFSVELSKMSSNKLKLNALKKKNQLVQKAI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIZ1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RIZ1 Antibody [NBP1-89644]

Immunocytochemistry/ Immunofluorescence: RIZ1 Antibody [NBP1-89644]

Immunocytochemistry/Immunofluorescence: RIZ1 Antibody [NBP1-89644] - Staining of human cell line U-251MG shows positivity in nucleus and golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Analysis in human cerebral cortex and liver tissues using NBP1-89644 antibody. Corresponding PRDM2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human liver shows very weak positivity in hepatocytes.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644]

Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
RIZ1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RIZ1 Antibody - BSA Free [NBP1-89644]

Chromatin Immunoprecipitation-exo-Seq: RIZ1 Antibody - BSA Free [NBP1-89644]

ChIP-Exo-Seq composite graph for Anti-PRDM2 (NBP1-89644) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for RIZ1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIZ1

RIZ1 (Retinoblastoma proteininteracting zinc finger), also known as PRDM2, is a nuclear protein containing 8 zinc finger motifs. RIZ1 contains a PR domain that may function as protein binding interface in the regulation of chromatin-mediated gene expression. RIZ 1 acts as a tumor suppressor, and RIZ 1 inactivation is common in many types of human cancers, including neuroendocrine, breast, liver, colon, skin, and lymphoid tumors. Similarly, RIZ1 over-expression in breast cancer cells caused cell cycle arrest in G2-M and apoptosis. RIZ1 is highly expressed in human retinoblastoma cells and at low levels in all other human cell lines.

Alternate Names

EC 2.1.1.43, GATA-3 binding protein G3B, GATA-3-binding protein G3B, KMT8PR domain-containing protein 2, Lysine N-methyltransferase 8, MTB-ZFHUMHOXY1, PR domain containing 2, with ZNF domain, PR domain zinc finger protein 2, retinoblastoma protein-binding zinc finger protein, Retinoblastoma protein-interacting zinc finger protein, RIZ1, RIZ2, RIZMTE-binding protein, Zinc finger protein RIZ, zinc-finger DNA-binding protein

Gene Symbol

PRDM2

Additional RIZ1 Products

Product Documents for RIZ1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RIZ1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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