RNA Helicase A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85266

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RNA Helicase A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Helicase A Antibody - BSA Free

Western Blot: RNA Helicase A Antibody [NBP1-85266]

Western Blot: RNA Helicase A Antibody [NBP1-85266]

Western Blot: RNA Helicase A Antibody [NBP1-85266] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody [NBP1-85266]

Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody [NBP1-85266]

Immunocytochemistry/Immunofluorescence: RNA Helicase A Antibody [NBP1-85266] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Western Blot: RNA Helicase A Antibody [NBP1-85266]

Western Blot: RNA Helicase A Antibody [NBP1-85266]

Western Blot: RNA Helicase A Antibody [NBP1-85266] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP1-85266] - Staining of human small intestine shows strong nuclear positivity in glandular cells.

Applications for RNA Helicase A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA Helicase A

DHX9, also known as ATP-dependent RNA helicase A, consists of a 141 kDa and a 24.3 kDa isoform and is involved in activating transcription and unwinding DNA and RNA helixes in the 3' to 5' direction. Current research is being conducted on diseases and disorders such as influenza, hepatitis, t-cell leukemia, astigmatism, keratitis, immunodeficiency, Werner syndrome, and arthritis. The protein interacts in mRNA splicing, mRNA processing, and gene expression pathways with HIST1H3A- HIST13E proteins.

Long Name

ATP-dependent RNA helicase A

Alternate Names

DDX9, DEAH box protein 9, DExH-box helicase 9, DHX9, EC 3.6.4.13, Leukophysin, LKP, NDH II, NDH2

Gene Symbol

DHX9

Additional RNA Helicase A Products

Product Documents for RNA Helicase A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RNA Helicase A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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