RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free

Novus Biologicals | Catalog # NBP2-59215

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, C. elegans, Zebrafish

Cited:

Human, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Knockdown Validated

Cited:

Chromatin Immunoprecipitation (ChIP), Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C.15200005

Format

BSA Free
View all RNA Polymerase II/POLR2A Primary Antibodies »

Product Specifications

Immunogen

The exact immunogen is propietary information.

Modification

p Ser2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.
Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S2p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.
ELISA: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Cross reactivity of the antibody directed against Pol IIS2p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS2p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S2 phosphorylation.
Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Applications for RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-2 ug/IP

Chromatin Immunoprecipitation Sequencing

1 - 2 ug/ChIP

ELISA

1:3000

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:1000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-59215 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free

Customer Reviews for RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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  • RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hep3B human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/01/2024
    WB RNA Pol II pSer2
    RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free NBP2-59215
  • RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005)
    Name: Wendy Lyu
    Application: Chromatin Immunoprecipitation
    Sample Tested: Human breast cancer cell lysate (MCF-7) and T47D human breast cancer cell line
    Species: Human
    Verified Customer | Posted 09/11/2020
    2ug NBP2-59215 antibody
    RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) - BSA Free NBP2-59215

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