Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 150 and 200 of human Ring Finger Protein 2 using the numbering given in entry NP_009143.1 (GeneID 6045).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit RNF2 Antibody - BSA Free (NBP1-49966) is a polyclonal antibody validated for use in IHC, WB, IP and ChIP. Anti-RNF2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RNF2 Antibody - BSA Free

Western Blot: RNF2 Antibody [NBP1-49966]

Western Blot: RNF2 Antibody [NBP1-49966]

Western Blot: RNF2 Antibody [NBP1-49966] - Detection of Human and Mouse RNF2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from K562 (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNF2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNF2 was also immunoprecipitated by rabbit anti-RNF2 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated RNF2 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966]

Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966]

Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RNF2 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector ImmPACT NovaRED
Immunohistochemistry: RNF2 Antibody [NBP1-49966]

Immunohistochemistry: RNF2 Antibody [NBP1-49966]

RNF2-Antibody-Immunohistochemistry-NBP1-49966-img0004.jpg
RNF2 Antibody

Immunohistochemistry: RNF2 Antibody [NBP1-49966] -

Immunohistochemistry: RNF2 Antibody [NBP1-49966] - Epigenetic remodelers were overexpressed in pancreatitis & pancreatic cancer(A) Immunohistochemistry staining of Bmi1, Ring1b & H2AK119ub in eight week old control mice, 48 hours & seven days after the last cerulein injection (n = 5). Representative images include boxes with high magnification to demonstrate nuclear staining of centroacinar (control) & ADM (48h) cells. Bar, 100 μm. (B) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. (C) Immunohistochemistry staining against Bmi1, Ring1b & H2AK119ub of pancreatic tissue from three week old KPC mice representing morphological normal tissue, from ten week old mice showing preinvasive ductal lesions & from 20 week old KPC mice demonstrating full PDAC (n = 5). High magnification images represent nuclear staining of centroacinar cells (3w-normal) or ADMs (10w-ADM). Bar, 100 μm. (D) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26716510), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RNF2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RNF2

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq]

Long Name

Ring Finger Protein 2

Alternate Names

DING, HIPI3, RING1B, RING2

Entrez Gene IDs

6045 (Human); 19821 (Mouse)

Gene Symbol

RNF2

UniProt

Additional RNF2 Products

Product Documents for RNF2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RNF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNF2 Antibody - BSA Free

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Protocols

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