RNF2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-49966
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 150 and 200 of human Ring Finger Protein 2 using the numbering given in entry NP_009143.1 (GeneID 6045).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RNF2 Antibody - BSA Free (NBP1-49966) is a polyclonal antibody validated for use in IHC, WB, IP and ChIP. Anti-RNF2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RNF2 Antibody - BSA Free
Western Blot: RNF2 Antibody [NBP1-49966]
Western Blot: RNF2 Antibody [NBP1-49966] - Detection of Human and Mouse RNF2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from K562 (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNF2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNF2 was also immunoprecipitated by rabbit anti-RNF2 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated RNF2 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966]
Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RNF2 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector ImmPACT NovaREDImmunohistochemistry: RNF2 Antibody [NBP1-49966]
RNF2-Antibody-Immunohistochemistry-NBP1-49966-img0004.jpgImmunohistochemistry: RNF2 Antibody [NBP1-49966] -
Immunohistochemistry: RNF2 Antibody [NBP1-49966] - Epigenetic remodelers were overexpressed in pancreatitis & pancreatic cancer(A) Immunohistochemistry staining of Bmi1, Ring1b & H2AK119ub in eight week old control mice, 48 hours & seven days after the last cerulein injection (n = 5). Representative images include boxes with high magnification to demonstrate nuclear staining of centroacinar (control) & ADM (48h) cells. Bar, 100 μm. (B) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. (C) Immunohistochemistry staining against Bmi1, Ring1b & H2AK119ub of pancreatic tissue from three week old KPC mice representing morphological normal tissue, from ten week old mice showing preinvasive ductal lesions & from 20 week old KPC mice demonstrating full PDAC (n = 5). High magnification images represent nuclear staining of centroacinar cells (3w-normal) or ADMs (10w-ADM). Bar, 100 μm. (D) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26716510), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for RNF2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RNF2
Long Name
Ring Finger Protein 2
Alternate Names
DING, HIPI3, RING1B, RING2
Gene Symbol
RNF2
UniProt
Additional RNF2 Products
Product Documents for RNF2 Antibody - BSA Free
Product Specific Notices for RNF2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for RNF2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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