RNF220 Antibody (5E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00055182-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E5

Format

Azide and BSA Free
View all RNF220 Primary Antibodies »

Product Specifications

Immunogen

FLJ10597 (NP_060620, 468 a.a. ~ 566 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Reactivity Notes

Human. Other species not tested.

Specificity

C1orf164 - hypothetical protein FLJ10597

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RNF220 Antibody (5E5) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RNF220 Antibody (5E5) [H00055182-M01]

Immunocytochemistry/ Immunofluorescence: RNF220 Antibody (5E5) [H00055182-M01]

Immunocytochemistry/Immunofluorescence: RNF220 Antibody (5E5) [H00055182-M01] - Analysis of monoclonal antibody to RNF220 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: RNF220 Antibody (5E5) [H00055182-M01] - Detection limit for recombinant GST tagged C1orf164 is approximately 0.03ng/ml as a capture antibody.

Applications for RNF220 Antibody (5E5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein on ELISA. GST alone used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF220

Alternate Names

C1orf164, chromosome 1 open reading frame 164, E3 ubiquitin-protein ligase RNF220, EC 6.3.2.-, ring finger protein 220FLJ10597

Entrez Gene IDs

55182 (Human)

Gene Symbol

RNF220

UniProt

NP_060620 (Human)

Additional RNF220 Products

Product Documents for RNF220 Antibody (5E5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RNF220 Antibody (5E5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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