ROS Antibody (OTI1F3) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-73932
![Western Blot: ROS Antibody (OTI1F3)Azide and BSA Free [NBP2-73932]](https://resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Azide-and-BSA-Free-Western-Blot-NBP2-73932-img0001.jpg "Western Blot: ROS Antibody (OTI1F3)Azide and BSA Free [NBP2-73932]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1F3
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 2126-2347 of human ROS1(NP_002935) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ROS Antibody (OTI1F3) - Azide and BSA Free
Western Blot: ROS Antibody (OTI1F3)Azide and BSA Free [NBP2-73932]
Western Blot: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS.![Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]](https://resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73932-img0004.jpg "Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]")
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932] - Analysis of Carcinoma Human lung tissue.![Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]](https://resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73932-img0002.jpg "Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]")
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932] - Analysis of Human carcinoma lung tissue.![Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]](https://resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73932-img0003.jpg "Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]")
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932] - Analysis of Carcinoma Human lung tissue.![Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]](https://resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73932-img0005.jpg "Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]")
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932]
Immunohistochemistry: ROS Antibody (OTI1F3) - Azide and BSA Free [NBP2-73932] - Staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (Left). The right is HELA cells as negative control (1:2000).Applications for ROS Antibody (OTI1F3) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:5000
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: ROS1
Long Name
ROS Proto-Oncogene 1, Receptor Tyrosine Kinase
Alternate Names
c-Ros-1, MCF3
Gene Symbol
ROS1
Additional ROS1 Products
Product Documents for ROS Antibody (OTI1F3) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ROS Antibody (OTI1F3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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