RPIP8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80855

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Mouse (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPIP8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKDLEAENRRLQLQLEEAAAQNQREKRELEGVILELQEQLTGLIPSDHAPLAQGSKELTTPLVNQWPSLGTLNGAEGASNSKL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPIP8 Antibody - BSA Free

Western Blot: RPIP8 Antibody [NBP1-80855]

Western Blot: RPIP8 Antibody [NBP1-80855]

Western Blot: RPIP8 Antibody [NBP1-80855] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855] - Staining of human liver.
Western Blot: RPIP8 Antibody [NBP1-80855]

Western Blot: RPIP8 Antibody [NBP1-80855]

Western Blot: RPIP8 Antibody [NBP1-80855] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-489
Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855] - Staining of human hippocampus shows strong extracellular positivity.
Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855] - Staining of human cerebral cortex, colon, hippocampus and liver using Anti-RUNDC3A antibody NBP1-80855 (A) shows similar protein distribution across tissues to independent antibody NBP2-68906 (B).
Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855]

Immunohistochemistry-Paraffin: RPIP8 Antibody [NBP1-80855] - Staining of human colon.
RPIP8 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: RPIP8 Antibody [NBP1-80855]

Immunocytochemistry/ Immunofluorescence: RPIP8 Antibody [NBP1-80855]

Staining of human cell line U-2 OS shows localization to vesicles.

Applications for RPIP8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPIP8

RPIP8 is a gene that codes for a protein that has four isoforms, with lengths of 446, 400, 405, and 364 amino acids long and weights of approximately 50, 45, 46, and 41 kDa, respectively. RPIP8 functions as an effector of RAP2A in neuronal cells. Current research is being done on diseases and disorders relating to this gene including dementia, ataxia, malaria, and neuronitis. RPIP8 has also been shown to have interactions with RAP2B, GFI1B, EIF3E, AP2M1, and AP1M1.

Alternate Names

Rap2-interacting protein 8, RAP2IPRUN domain-containing protein 3A, RPIP-8, RPIP8RaP2 interacting protein 8, RUN domain containing 3A

Entrez Gene IDs

10900 (Human)

Gene Symbol

RUNDC3A

UniProt

Q59EK9 (Human)

Additional RPIP8 Products

Product Documents for RPIP8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPIP8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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