RPL7A Antibody

Novus Biologicals | Catalog # NB100-2277

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RPL7A Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 215 and the C-terminus (residue 266) of human Ribosomal Protein L7a using the numbering given in entry NP_000963.1 (GeneID 6130).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL7A Antibody

Western Blot: RPL7A Antibody [NB100-2277]

Western Blot: RPL7A Antibody [NB100-2277]

Western Blot: RPL7A Antibody [NB100-2277] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-rpL7a/SURF3 antibody NB100-2277 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: RPL7A Antibody [NB100-2277]

Immunohistochemistry-Paraffin: RPL7A Antibody [NB100-2277]

Immunohistochemistry-Paraffin: RPL7A Antibody [NB100-2277] - Sample: FFPE sections of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- rpL7a (SURF3) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: RPL7A Antibody [NB100-2277]

Western Blot: RPL7A Antibody [NB100-2277]

Western Blot: RPL7A Antibody [NB100-2277] - Detection of Human rpL7a (SURF3) on HeLa whole cell lysate using NB100-2277.

Applications for RPL7A Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPL7A

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

60S ribosomal protein L7a, PLA-X polypeptide, ribosomal protein L7a, SURF-3, SURF3surfeit 3, Surfeit locus protein 3, thyroid hormone receptor uncoupling protein, TRUP

Entrez Gene IDs

6130 (Human)

Gene Symbol

RPL7A

UniProt

NP_000963.1 (Human)

Additional RPL7A Products

Product Documents for RPL7A Antibody

Certificate of Analysis

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Product Specific Notices for RPL7A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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