RPL8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48827

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL8 Antibody - BSA Free

Western Blot: RPL8 Antibody [NBP2-48827]

Western Blot: RPL8 Antibody [NBP2-48827]

Western Blot: RPL8 Antibody [NBP2-48827] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RPL8 antibody. Remaining relative intensity is presented.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human cerebellum, fallopian tube, pancreas and skin using Anti-RPL8 antibody NBP2-48827 (A) shows similar protein distribution across tissues to independent antibody NBP2-47297 (B).
Western Blot: RPL8 Antibody [NBP2-48827]

Western Blot: RPL8 Antibody [NBP2-48827]

Western Blot: RPL8 Antibody [NBP2-48827] - Analysis using Anti-RPL8 antibody NBP2-48827 (A) shows similar pattern to independent antibody NBP2-47297 (B).
Immunohistochemistry: RPL8 Antibody [NBP2-48827]

Immunohistochemistry: RPL8 Antibody [NBP2-48827]

Immunohistochemistry: RPL8 Antibody [NBP2-48827] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human colon.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human lymph node.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human liver.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827]

Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-48827] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.

Applications for RPL8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPL8

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

ribosomal protein L8,60S ribosomal protein L8

Gene Symbol

RPL8

Additional RPL8 Products

Product Documents for RPL8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPL8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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