RPMI 1640, 25 mM HEPES, GlutaminePlus

Atlanta Biologicals media and balanced salt solutions are now sold under the R&D Systems brand
Catalog # Availability Size / Price Qty

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

RPMI 1640, 25 mM HEPES, GlutaminePlus
1 Image
Product Details

RPMI 1640, 25 mM HEPES, GlutaminePlus Summary

RPMI 1640 Medium with 25 mM HEPES buffer and GlutaminePlus.

What is RPMI?

RPMI 1640 Medium was developed for culturing human leukocytes and other blood cells. It contains several additional components when compared to MEM and DMEM. RPMI 1640 is now used for a range of mammalian cells in suspension and monolayer culture. RPMI is manufactured as enriched medium with or without L-glutamine with a stable L-glutamine substitute, with or without HEPES. RPMI 1640 requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.

Each lot of RPMI 1640 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of RPMI 1640 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. RPMI 1640 is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the datasheet.


0.2 μm filtered
Sterility Testing
Shipping Conditions
2 - 8 °C, protected from light
Cell Culture
Country of Origin


For research use only. Not for diagnostic use.

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all FAQs

Reviews for RPMI 1640, 25 mM HEPES, GlutaminePlus

There are currently no reviews for this product. Be the first to review RPMI 1640, 25 mM HEPES, GlutaminePlus and earn rewards!

Have you used RPMI 1640, 25 mM HEPES, GlutaminePlus?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review