RPSA Antibody (RPSA/2699)

Novus Biologicals | Catalog # NBP2-79720

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RPSA/2699
View all RPSA Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human RPSA protein (Uniprot: P08865)

Localization

Cell Surface, Cytoplasmic, Nuclear

Marker

Marker of Metastatic Potential

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml. (NBP2-79854)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for RPSA Antibody (RPSA/2699)

Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720]

Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720]

Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).
Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of paraformaldehyde-Raji cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with RPSA Antibody (RPSA/2699).
Flow Cytometry: RPSA Antibody (RPSA/2699) [NBP2-79720]

Flow Cytometry: RPSA Antibody (RPSA/2699) [NBP2-79720]

Flow Cytometry: RPSA Antibody (RPSA/2699) [NBP2-79720] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of HeLa cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RPSA Antibody (RPSA/2699).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RPSA Antibody (RPSA/2699).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with RPSA Antibody (RPSA/2699).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720]

Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Tonsil stained with RPSA Antibody (RPSA/2699).
Protein Array: RPSA Antibody (RPSA/2699) [NBP2-79720]

Protein Array: RPSA Antibody (RPSA/2699) [NBP2-79720]

Protein Array: RPSA Antibody (RPSA/2699) [NBP2-79720] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RPSA Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for RPSA Antibody (RPSA/2699)

Application
Recommended Usage

Flow Cytometry

1-2 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: RPSA

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 37LRPNEM/1CHD4, 67 kDa laminin receptor, 67kD, ribosomal protein SA, 67LR, Colon carcinoma laminin-binding protein, LAMBR37 kDa laminin receptor, laminin binding protein, Laminin receptor 1, laminin receptor 1 (67kD, ribosomal protein SA), Laminin-binding protein precursor p40, LamR, LAMR 1, LAMR140S ribosomal protein SA, LBP, LBP/p40, LRP, LRP/LR, Multidrug resistance-associated protein MGr1-Ag, p40, ribosomal protein SA

Gene Symbol

RPSA

UniProt

P08865 (Human)

Additional RPSA Products

Product Documents for RPSA Antibody (RPSA/2699)

Certificate of Analysis

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Product Specific Notices for RPSA Antibody (RPSA/2699)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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