Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPTOR Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Regulatory Associated Protein of mTOR using the numbering given in entry AAM09075.1 (GeneID 57521).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPTOR Antibody - BSA Free

Western Blot: RPTOR Antibody [NB100-766]

Western Blot: RPTOR Antibody [NB100-766]

Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RPTOR Antibody [NB100-766]

Immunoprecipitation: RPTOR Antibody [NB100-766]

Immunoprecipitation: RPTOR Antibody [NB100-766] - Detection of human Raptor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Raptor antibody NB100-766 (lot 3) used for IP at 6 ug per reaction. Raptor was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-Raptor antibody NB100-331 For blotting immunoprecipitated Raptor, NB100-766 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for RPTOR Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 -10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPTOR

Raptor (regulatory associated protein of mTOR) is a 150 kDa mTOR (Target of Rapamycin) binding protein. It plays a positive role in nutrient-stimulated signaling and cell size maintenance.

Long Name

Regulatory Associated Protein of mTOR

Alternate Names

KIAA1303, Raptor

Entrez Gene IDs

57521 (Human); 74370 (Mouse)

Gene Symbol

RPTOR

UniProt

NP_065812.1 (Human)

Additional RPTOR Products

Product Documents for RPTOR Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RPTOR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Interferons

Citations for RPTOR Antibody - BSA Free

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