RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20098

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-30C-18

Format

Azide and BSA Free
View all RRM2 Primary Antibodies »

Product Specifications

Immunogen

The KLH-conjugated peptide "IEQEFLTEALPVK" derived from Ribonucleoside-diphosphate reductase subunit M2 (RRM2). RRM2 provides precursors required for DNA synthesis.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] -

Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

Flow Cytometry: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] -

Flow Cytometry: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-30C-18 (NBP3-20098, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] -

Immunocytochemistry/Immunofluorescence: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-30C-18 (NBP3-20098) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20098, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Ab can be used in immunoMRM.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: RRM2

Ribonucleotide reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. It is composed of 2 non-identical subunits, proteins M1 and M2. Synthesis of M2 is regulated in a cell-cycle dependent fashion.

Long Name

Ribonucleotide Reductase Regulatory Subunit M2

Alternate Names

C2orf48, FLJ25102, RR2M

Gene Symbol

RRM2

Additional RRM2 Products

Product Documents for RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free and earn rewards!

Have you used RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies