RSK1 Antibody (23GB2080)

Novus Biologicals | Catalog # NBP3-50996

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2080
View all RSK1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human RSK1 p90 (Uniprot: Q15418)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RSK1 Antibody (23GB2080)

RSK1 Antibody (23GB2080)

Immunocytochemistry/ Immunofluorescence: RSK1 Antibody (23GB2080)[NBP3-50996] -

Immunocytochemical staining of Hela cells with RSK1 antibody (1:1,000). Nuclei were stained blue with DAPI; RSK1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
RSK1 Antibody (23GB2080)

Flow Cytometry: RSK1 Antibody (23GB2080)[NBP3-50996] -

Flow cytometric analysis of RSK1expression in HeLa cells using RSK1 antibody (1:2,000). Green, isotype control; red, RPS6KA1.
RSK1 Antibody (23GB2080)

Western Blot: RSK1 Antibody (23GB2080)[NBP3-50996] -

Western blotting analysis using RSK1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
RSK1 Antibody (23GB2080)

Western Blot: RSK1 Antibody (23GB2080)[NBP3-50996] -

Western blotting analysis using RSK1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for RSK1 Antibody (23GB2080)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RSK1

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Gene Symbol

RPS6KA1

Additional RSK1 Products

Product Documents for RSK1 Antibody (23GB2080)

Certificate of Analysis

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Product Specific Notices for RSK1 Antibody (23GB2080)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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