RXR alpha/NR2B1 Antibody

Novus Biologicals | Catalog # NB100-1466

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
Loading...

Product Specifications

Immunogen

Peptide with sequence CQVNSSLTSPTGRGSM corresponding to internal region according to NP_002948.1.

Specificity

This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RXR alpha/NR2B1 Antibody

Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466]

Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466]

Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Western blot analysis of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). 35 ug protein in RIPA buffer. Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody [NB100-1466]

Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody [NB100-1466]

Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody [NB100-1466] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: RXR alpha/NR2B1 Antibody [NB100-1466]

Flow Cytometry: RXR alpha/NR2B1 Antibody [NB100-1466]

Flow Cytometry: RXR alpha/NR2B1 Antibody [NB100-1466] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
RXR alpha/NR2B1 Antibody

Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] -

Determination of Clover-PPAR gamma 1, mRuby2-RXR alpha and mRuby2-RXR alpha delta 414-462 protein expression. Graphical scheme of the structure of the full length human PPAR gamma 1 protein (A); the full length human RXR alpha protein (B) and the human RXR alpha deletion construct with a C-terminal absence of the sequence AA 414-462 (C). PPAR gamma 1 was N-terminally labeled with the green fluorophore Clover and both of the two RXR alpha constructs N-terminally with the red fluorophore mRuby2. Clover-PPAR gamma 1, mRuby2-RXR alpha and mRuby2-RXR alpha delta 414-462 protein expressions are shown in total HEK293T cell lysates visualized by Western blot analysis against human PPAR gamma (D), GFP (E), human RXR alpha (F) and tRFP (G). Images are representative of experiments which were performed at least three times. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31534496), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RXR alpha/NR2B1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 55-60 kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8 kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RXR alpha/NR2B1

Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).

Long Name

Retinoid X Receptor, alpha

Alternate Names

NR2B1, RXRA

Entrez Gene IDs

6256 (Human)

Gene Symbol

RXRA

Additional RXR alpha/NR2B1 Products

Product Documents for RXR alpha/NR2B1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RXR alpha/NR2B1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RXR alpha/NR2B1 Antibody

Customer Reviews for RXR alpha/NR2B1 Antibody

There are currently no reviews for this product. Be the first to review RXR alpha/NR2B1 Antibody and earn rewards!

Have you used RXR alpha/NR2B1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...