S100A13 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86039

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all S100A13 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELEESIETVVTTFFTFARQEGRKDSLSVNEFKELVTQQLPHLLKDVGSLDEKMKSLDVNQDSELKFNEYWRLIGELAKEIRKKKDLKI

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24722184). Mouse (82%%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S100A13 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: S100A13 Antibody [NBP1-86039]

Immunocytochemistry/ Immunofluorescence: S100A13 Antibody [NBP1-86039]

Immunocytochemistry/Immunofluorescence: S100A13 Antibody [NBP1-86039] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: S100A13 Antibody [NBP1-86039]

Immunohistochemistry-Paraffin: S100A13 Antibody [NBP1-86039]

Immunohistochemistry-Paraffin: S100A13 Antibody [NBP1-86039] - Staining of human thyroid gland shows strong cytoplasmic and nuclear positivity in glandular cells.
S100A13 Antibody

Western Blot: S100A13 Antibody [NBP1-86039] -

Western blot analyses of ANXA3, CMBL & S100A13 expression.Depleted cyst fluids from cPTCs & benign lesions are shown together with total protein extract from A549 cells. Protein sizes are indicated to the right in kDa. Ponceau S solution used as loading control is shown below. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0126472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
S100A13 Antibody - BSA Free Western Blot: S100A13 Antibody - BSA Free [NBP1-86039]

Western Blot: S100A13 Antibody - BSA Free [NBP1-86039]

Analysis in human cell line MCF-7.
S100A13 Antibody - BSA Free Western Blot: S100A13 Antibody - BSA Free [NBP1-86039]

Western Blot: S100A13 Antibody - BSA Free [NBP1-86039]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for S100A13 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC, WB reported in scientific literature (PMID: 24722184). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A13

S100A13 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Long Name

S100 Calcium Binding Protein

Alternate Names

S100 calcium binding protein A13, S100 calcium-binding protein A13protein S100-A13

Gene Symbol

S100A13

Additional S100A13 Products

Product Documents for S100A13 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for S100A13 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for S100A13 Antibody - BSA Free

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Protocols

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