S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-79899

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CPTC-S100A2-2

Format

Azide and BSA Free
View all S100A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human S100A2 protein (Uniprot: P29034)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

11.12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79764).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free

Western Blot: S100A2 Antibody (CPTC-S100A2-2)Azide and BSA Free [NBP2-79899]

Western Blot: S100A2 Antibody (CPTC-S100A2-2)Azide and BSA Free [NBP2-79899]

Western Blot: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899] - Western Blot Analysis of A431 cell lysate using S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2)
Immunocytochemistry/ Immunofluorescence: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Immunocytochemistry/ Immunofluorescence: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Immunocytochemistry/Immunofluorescence: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899] - Immunofluorescence Analysis of A549 cells labeling S100A2 with S100A2 Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)
Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899] - Formalin-fixed, paraffin-embedded human Placenta stained with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).
Flow Cytometry: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Flow Cytometry: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Flow Cytometry: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899] - Flow Cytometric Analysis of trypsinised HeLa cells. S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899] - Formalin-fixed, paraffin-embedded human Skin stained with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).
Protein Array: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Protein Array: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899]

Protein Array: S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free [NBP2-79899] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A2 Antibody (CPTC-S100A2-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: S100A2

S100 alpha 2 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer.

Long Name

S100 Calcium Binding Protein A2

Alternate Names

CAN19, S100L

Gene Symbol

S100A2

Additional S100A2 Products

Product Documents for S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free and earn rewards!

Have you used S100A2 Antibody (CPTC-S100A2-2) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies