SAM68 Antibody

Novus Biologicals | Catalog # NBP2-20254

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SAM68 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAM68. The exact sequence is proprietary.

Reactivity Notes

Chicken (87%).

Localization

Nucleus; Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAM68 Antibody

Western Blot: SAM68 Antibody [NBP2-20254]

Western Blot: SAM68 Antibody [NBP2-20254]

Western Blot: SAM68 Antibody [NBP2-20254] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SAM68 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SAM68 Antibody [NBP2-20254]

Immunocytochemistry/ Immunofluorescence: SAM68 Antibody [NBP2-20254]

Immunocytochemistry/Immunofluorescence: SAM68 Antibody [NBP2-20254] - SAM68 antibody detects SAM68 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SAM68 stained by SAM68 antibody diluted at 1:2000. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-20254]

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-20254]

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-20254] - SAM68 antibody detects SAM68 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. SAM68 stained by SAM68 antibody diluted at 1:500.
Western Blot: SAM68 Antibody [NBP2-20254]

Western Blot: SAM68 Antibody [NBP2-20254]

Western Blot: SAM68 Antibody [NBP2-20254] - Sample (30 ug of whole cell lysate) A: Jurakt 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-20254]

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-20254]

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-20254] - Paraffin-embedded rat fore brain. SAM68 antibody [N2C3] dilution: 1:500.
SAM68 Antibody

Western Blot: SAM68 Antibody [NBP2-20254] -

SAM68 antibody [N2C3] detects SAM68 protein by western blot analysis.
A. 30 ug PC-12 whole cell extract
B. 30 ug Rat2 whole cell extract
7.5 % SDS-PAGE
SAM68 antibody [N2C3] (NBP2-20254) dilution: 1:3000

Applications for SAM68 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAM68

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export Function: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase

Long Name

Src-associated in Mitosis 68 kDa Protein

Alternate Names

KHDRBS1, p62, p68

Gene Symbol

KHDRBS1

UniProt

Q07666

Additional SAM68 Products

Product Documents for SAM68 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SAM68 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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