SAMHD1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-12776
![Western Blot: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Western-Blot-NBP2-12776-img0007.jpg "Western Blot: SAMHD1 Antibody [NBP2-12776]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 576 and 626 of human SAM Domain and HD Domain 1 using the numbering given in entry NP_056289.2 (GeneID 25939).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SAMHD1 Antibody - BSA Free
Western Blot: SAMHD1 Antibody [NBP2-12776]
Western Blot: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Immunohistochemistry-NBP2-12776-img0003.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]")
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-12776-img0005.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]")
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunoprecipitation: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Immunoprecipitation-NBP2-12776-img0006.jpg "Immunoprecipitation: SAMHD1 Antibody [NBP2-12776]")
Immunoprecipitation: SAMHD1 Antibody [NBP2-12776]
Immunoprecipitation: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 (lot NBP2-12776-1) used for IP at 6 ug/mg lysate. SAMHD1 was also immunoprecipitated by another rabbit anti-SAMHD1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated SAMHD1, NBP2-12776 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.Applications for SAMHD1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:5000
Immunohistochemistry-Paraffin
1:500 - 1:5000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SAMHD1
Long Name
SAM Domain and HD Domain 1
Alternate Names
AGS5, CHBL2, DCIP, HDDC1, MOP-5, SBBI88
Gene Symbol
SAMHD1
UniProt
Additional SAMHD1 Products
Product Documents for SAMHD1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SAMHD1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for SAMHD1 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review SAMHD1 Antibody - BSA Free and earn rewards!
Have you used SAMHD1 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...