SAMHD1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-12776
![Western Blot: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Western-Blot-NBP2-12776-img0007.jpg "Western Blot: SAMHD1 Antibody [NBP2-12776]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 576 and 626 of human SAM Domain and HD Domain 1 using the numbering given in entry NP_056289.2 (GeneID 25939).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SAMHD1 Antibody - BSA Free
Western Blot: SAMHD1 Antibody [NBP2-12776]
Western Blot: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Immunohistochemistry-NBP2-12776-img0003.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]")
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-12776-img0005.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]")
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776]
Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunoprecipitation: SAMHD1 Antibody [NBP2-12776]](https://resources.rndsystems.com/images/products/SAMHD1-Antibody-Immunoprecipitation-NBP2-12776-img0006.jpg "Immunoprecipitation: SAMHD1 Antibody [NBP2-12776]")
Immunoprecipitation: SAMHD1 Antibody [NBP2-12776]
Immunoprecipitation: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 (lot NBP2-12776-1) used for IP at 6 ug/mg lysate. SAMHD1 was also immunoprecipitated by another rabbit anti-SAMHD1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated SAMHD1, NBP2-12776 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.Applications for SAMHD1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:5000
Immunohistochemistry-Paraffin
1:500 - 1:5000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SAMHD1
Long Name
SAM Domain and HD Domain 1
Alternate Names
AGS5, CHBL2, DCIP, HDDC1, MOP-5, SBBI88
Gene Symbol
SAMHD1
UniProt
Additional SAMHD1 Products
Product Documents for SAMHD1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SAMHD1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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