SARS-CoV-2 3CL Protease Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-07062
![Western Blot: SARS-CoV-2 3CL Protease AntibodyBSA Free [NBP3-07062]](https://resources.rndsystems.com/images/products/SARS-CoV-2-3CL-Protease-Antibody-Western-Blot-NBP3-07062-img0001.jpg "Western Blot: SARS-CoV-2 3CL Protease AntibodyBSA Free [NBP3-07062]")
Key Product Details
Species Reactivity
Validated:
SARS-CoV-2
Predicted:
SARS-CoV (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 3CL Protease Antibody - BSA Free
Western Blot: SARS-CoV-2 3CL Protease AntibodyBSA Free [NBP3-07062]
Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein. Antibodies: SARS-CoV-2 3CL Protease, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml![Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]](https://resources.rndsystems.com/images/products/SARS-CoV-2-3CL-Protease-Antibody-Immunohistochemistry-Paraffin-NBP3-07062-img0002.jpg "Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]")
Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]
Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 3CL protease antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.![ELISA: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]](https://resources.rndsystems.com/images/products/SARS-CoV-2-3CL-Protease-Antibody-ELISA-NBP3-07062-img0003.jpg "ELISA: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]")
ELISA: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]
ELISA: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Antibodies: SARS-CoV-2 3CL Protease antibody. A direct ELISA was performed using SARS-CoV-2 3CL Protease recombinant protein as coating antigen and SARS-CoV-2 3CL Protease antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mLApplications for SARS-CoV-2 3CL Protease Antibody - BSA Free
Application
Recommended Usage
ELISA
1 ug/mL
Immunohistochemistry
0.5 ug/mL
Immunohistochemistry-Paraffin
0.5 ug/mL
Western Blot
0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: 3CL Protease
Long Name
3CL Protease
Alternate Names
Main Protease, Mpro
Gene Symbol
ORF1ab
Additional 3CL Protease Products
Product Documents for SARS-CoV-2 3CL Protease Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SARS-CoV-2 3CL Protease Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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