SARS-CoV-2 Membrane Protein Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-07059

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Predicted:

SARS-CoV (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SARS-CoV-2 Membrane Protein Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) Membrane protein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 Membrane Protein Antibody - BSA Free

Western Blot: SARS-CoV-2 Membrane Protein AntibodyBSA Free [NBP3-07059]

Western Blot: SARS-CoV-2 Membrane Protein AntibodyBSA Free [NBP3-07059]

Western Blot: SARS-CoV-2 Membrane Protein Antibody [NBP3-07059] - Western Blot Validation with SARS-CoV-2 Membrane Protein. Loading: 30 ng per lane of SARS-CoV-2 Membrane Protein. Antibodies: SARS-CoV-2 Membrane Protein (NBP3-07059), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/ml and Lane 2: 2 ug/ml
ELISA: SARS-CoV-2 Membrane Protein Antibody - BSA Free [NBP3-07059]

ELISA: SARS-CoV-2 Membrane Protein Antibody - BSA Free [NBP3-07059]

ELISA: SARS-CoV-2 Membrane Protein Antibody [NBP3-07059] - ELISA Validation with SARS-CoV-2 Membrane Protein. Antibodies: SARS-CoV-2 Membrane Protein Antibody (NBP3-07059). A direct ELISA was performed using SARS-CoV-2 Membrane Protein as coating antigen and the anti-SARS-CoV-2 Membrane Protein antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/ml to 3000 ng/ml.
SARS-CoV-2 Membrane Protein Antibody - BSA Free

Immunohistochemistry: SARS-CoV-2 Membrane Protein Antibody - BSA Free [NBP3-07059] -

Immunohistochemistry: SARS-CoV-2 Membrane Protein Antibody - BSA Free [NBP3-07059] - Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Membrane antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Applications for SARS-CoV-2 Membrane Protein Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry

0.5 ug/ml

Western Blot

0.25 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SARS-CoV-2 Membrane Protein

The SARS-CoV-2 Membrane protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). The membrane protein is the most abundant of the four structural proteins, which also includes the envelope protein, spike protein, and nucleocapsid protein (1,2). The membrane protein is synthesized as a 222 amino acid protein with a theoretical molecular weight of 25.1 kDa (2,3). Furthermore, the membrane protein of SARS-CoV-2 has 90.5% sequence identity and 98.2% sequence similarity to that of the membrane protein of SARS-CoV (2). Structurally, the membrane protein is a type III transmembrane glycoprotein containing a glycosylated N-terminal ectodomain, three transmembrane domains, and a large C-terminal that extends into the viral particle (2,4). The membrane protein exists as a dimer and is either in long or compact conformation, which contributes to the curvature of the viral membrane (2,4). The main functions of the membrane protein are for viral assembly and contributing to the shape and structural integrity of the viral envelope (1,2). Additional functions show a role in inducing apoptosis as well as interactions with the spike and nucleocapsid proteins (1,2). The membrane-spike protein interaction is required for maintaining the spike protein within the ER-Golgi intermediate compartment (1). The membrane protein binding with the nucleocapsid protein is responsible for nucleocapsid stabilization and viral assembly (1).

References

1. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

2. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

3. Uniprot (P0DTC5)

4. J Alsaadi, E. A., & Jones, I. M. (2019). Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144

Alternate Names

2019-nCoV M protein, 2019-nCoV Membrane Protein, COVID-19 E1 Glycoprotein, COVID-19 Matrix glycoprotein, COVID-19 Membrane, COVID-19 Membrane glycoprotein, E1 glycoprotein, Human coronavirus Membrane Protein, Matrix glycoprotein, Membrane glycoprotein, Membrane Protein, SARS-CoV-2, SARS-CoV-2 E1 Glycoprotein, SARS-CoV-2 M Protein, SARS-CoV-2 Matrix glycoprotein, SARS-CoV-2 Membrane glycoprotein, SARSCoV2 Membrane Protein, Severe Acute Respiratory Syndrome Coronavirus 2

Gene Symbol

M

Additional SARS-CoV-2 Membrane Protein Products

Product Documents for SARS-CoV-2 Membrane Protein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 Membrane Protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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