Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunohistochemistry, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SARS-CoV-2 nsp13 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against a peptide corresponding to 14 amino acids near the center of the SARS-CoV-2 (COVID-19) NSP13 (Helicase) protein. The immunogen is located between 440-490 amino acids of SARS-CoV-2 (COVID-19) NSP13 (Helicase).

Reactivity Notes

Predicted reactivity based on immunogen sequence: SARS-CoV NSP13 (Helicase) protein: (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 nsp13 Antibody - BSA Free

Immunohistochemistry: SARS-CoV-2 nsp13 Antibody - BSA Free [NBP3-07056]

Immunohistochemistry: SARS-CoV-2 nsp13 Antibody - BSA Free [NBP3-07056]

Immunohistochemistry: SARS-CoV-2 nsp13 Antibody [NBP3-07056] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP13 antibody ( 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees celsius. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP13 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA: SARS-CoV-2 nsp13 Antibody - BSA Free [NBP3-07056]

ELISA: SARS-CoV-2 nsp13 Antibody - BSA Free [NBP3-07056]

ELISA: SARS-CoV-2 nsp13 Antibody [NBP3-07056] - Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody. A direct ELISA was performed using SARS-CoV-2 NSP13 recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.

Applications for SARS-CoV-2 nsp13 Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry

0.5 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp13

SARS-CoV-2 Nonstructural Protein 13 (NSP13) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP13 is 601 amino acids (aa) with a theoretical molecular weight of 66.9 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP13 has 99.8% sequence identity and 100% sequence similarity, with only one amino acid residue difference between the two proteins (1). NSP13 is suggested to have helicase activity and unwinds RNA (1,3). Furthermore, NSP13 is suggested to interact with NSP12 and through protein-protein interactions, NSP12 enhances NSP13's helicase activity (1,3,4). Additionally, a SARS-CoV-2 interactome study reveals NSP13's involvement in several processes including transcriptional regulation, Golgi organization, protein kinase A signaling, and centrosome complex formation (2). Given its role in centrosome organization, NSP13 is a possible molecular link for the loss of smell, called anosmia, due to the olfactory cilia defects that can occur because of COVID-19 infection (5). NSP13 also interacts with innate immune pathways including the interferon pathway and NF-kappaB pathway (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M.,... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Romano, M., Ruggiero, A., Squeglia, F., Maga, G., & Berisio, R. (2020). A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. https://doi.org/10.3390/cells9051267

5. Li, W., Li, M., & Ou, G. (2020). COVID-19, cilia, and smell. The FEBS journal. https://doi.org/10.1111/febs.15491

Alternate Names

helicase, Non-structural protein 13

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp13 Products

Product Documents for SARS-CoV-2 nsp13 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 nsp13 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SARS-CoV-2 nsp13 Antibody - BSA Free

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Protocols

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