SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25666

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1722 expressed in HEK293

Format

Azide and BSA Free
View all SARS-CoV-2 ORF3a Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 ORF3a. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] -

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:500. Blue: Fluoroshield with DAPI.
SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

Immunohistochemistry-Paraffin: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] -

Immunohistochemistry-Paraffin: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunohistochemical analysis. Sample: Paraffin-embedded mock and SARS-CoV-2 (COVID-19) ORF3a transfected 293T. Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

Western Blot: SARS-CoV-2 ORF3a Antibody (HL1722) [NBP3-25666] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 ORF3a

SARS-CoV-2 Open Reading Frame 3a (ORF3a) is one of the nine downstream accessory protein open reading frames of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 ORF3a is 475 amino acids (aa) with a theoretical molecular weight of 31 kDa (1, 2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's ORF3a has 72% sequence identity and 90% sequence similarity (3). Structurally, ORF3a is a homotetramer that is comprised of two non-covalently linked homodimers (2).

The SARS-CoV-2 ORF3a protein encodes for a Ca2+ ion channel that functions in NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) inflammasome activation (3, 4). The ORF3a ion channel also functions in viral particle release as well as apoptotic and necrotic cell death (5). SARS-CoV-2 ORF3a interacts with the inflammasome component TRAF3 (TNF Receptor Associated Factor 3) which activates ASC (apoptosis-associated speck-like protein contain CARD) ubiquitination and, in-turn, stimulates caspase-1 activation and IL-1beta (interleukin-1-beta) production and maturation (3, 4). IL-1beta activation results in cytokine production and the overproduction of cytokines referred to as a cytokine storm, a hallmark of COVID-19 (4). Additionally, high throughput screening experiments have revealed the SARS-CoV-2 ORF3a binds to the human heme oxygenase (HMOX1) protein (5). HMOX1 has a role in reducing inflammation and tissue damage, both features of SARS-CoV-2 infection (5). The ORF3a-HMOX1 interaction will be worth exploring for its role in innate immune response and as a potential therapeutic target for COVID-19 (5).

Alternative names for SARS-CoV-2 ORF3a includes 2019-nCoV ORF3a protein, COVID-19 ORF3a, Human coronavirus ORF3a protein, ORF3a protein, SARS-CoV-2 accessory protein 3a, SARS-CoV-2 protein 3a, SARS-CoV-2 protein U274, and SARS-CoV-2 protein X1.

References

1. Michel, C. J., Mayenr, C., Poch, O., & Thompson, J. D. (2020). Characterization of accessory genes in coronavirus genomes. Virology journal. https://doi.org/10.1186/s12985-020-01402-12. UniProt (P0DTC3)

3. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The protein journal. https://doi.org/10.1007/s10930-020-09901-4

4. Oladele, J. O., Ajayi, E. I., Oyeleke, O. M., Oladele, O. T., Olowookere, B. D., Adeniyi, B. M., Oyewole, O. I., & Oladiji, A. T. (2020). A systematic review on COVID-19 pandemic with special emphasis on curative potentials of Nigeria based medicinal plants. Heliyon. https://doi.org/10.1016/j.heliyon.2020.e04897

5. Batra, N., De Souza, C., Batra, J., Raetz, A. G., & Yu, A. M. (2020). The HMOX1 Pathway as a Promising Target for the Treatment and Prevention of SARS-CoV-2 of 2019 (COVID-19). International journal of molecular sciences. https://doi.org/10.3390/ijms21176412

Alternate Names

2019-nCoV ORF3a, 2019-nCoV ORF3a Protein, COVID-19 ORF3a, Human coronavirus ORF3a Protein, ORF3a protein, SARS-CoV-2, SARS-CoV-2 Accessory protein 3a, SARSCoV2 ORF3a Protein, SARS-CoV-2 ORF3a Protein, SARS-CoV-2 Protein 3a, SARS-CoV-2 Protein U274, SARS-CoV-2 Protein X1, Severe Acute Respiratory Syndrome Coronavirus 2

Gene Symbol

ORF3a

Additional SARS-CoV-2 ORF3a Products

Product Documents for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free and earn rewards!

Have you used SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies