Sars Membrane Protein Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56569

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

SARS-CoV

Predicted:

SARS-CoV-2 (93%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Sars Membrane Protein Primary Antibodies »

Product Specifications

Immunogen

The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 195-210 (YSRYRIGNYKLNTDH) from the M (Membrane protein) for the Human SARS coronavirus (Genbank accession no. YP_009724393.1)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (93%)

Specificity

The NCBI accession number of the SARS matrix protein is NP_828855.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sars Membrane Protein Antibody - BSA Free

Western Blot: Sars Membrane Protein Antibody [NB100-56569]

Western Blot: Sars Membrane Protein Antibody [NB100-56569]

Western Blot: Sars Membrane Protein Antibody [NB100-56569] - Western blot analysis of Sars Membrane Protein in (A) recombinant fusion protein containing amino acids 195-210 and (B) fusion partner without these amino acids, using NB100-56569 at 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: Sars Membrane Protein Antibody [NB100-56569]

Immunocytochemistry/ Immunofluorescence: Sars Membrane Protein Antibody [NB100-56569]

Immunocytochemistry/Immunofluorescence: Sars Membrane Protein Antibody [NB100-56569] - Vero cells infected with SARS-CoV-2. Blue: DAPI; Green: SARS-CoV-2 membrane protein (NB100-56569); Magenta: SARS-CoV-2 nuclecapsid protein. Image from verified customer review.

Applications for Sars Membrane Protein Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported by customer review

Western Blot

0.5-2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-56569 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sars Membrane Protein

A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The M protein (Membrane protein, Matrix protein) is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein and the nucleocapsid protein. Coronaviruses have four important viral genes with different structural proteins: a spike glycoprotein (S), a small envelope protein (E), a matrix glycoprotein (M), and a nucleocapsid protein (N).

Alternate Names

E1 glycoprotein, Matrix glycoprotein, Membrane glycoprotein, SARS coronavirus M protein, SARS coronavirus Membrane protein, SARS CoV, SARS CoV M glycoprotein, SARS CoV Matrix protein, Sars M, Sars Matrix Protein, SARSCoV, SARS-CoV M protein, SARS-CoV Membrane protein, Severe acute respiratory syndrome

Entrez Gene IDs

AFR58746.1 (Virus)

UniProt

P59596 (Virus)

Additional Sars Membrane Protein Products

Product Documents for Sars Membrane Protein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Sars Membrane Protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Sars Membrane Protein Antibody - BSA Free

Customer Reviews for Sars Membrane Protein Antibody - BSA Free (1)

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Customer Images

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  • Sars Membrane Protein Antibody
    Name: Luca Mascheroni
    Application: Immunocytochemistry
    Sample Tested: Vero cell
    Species: Monkey
    Verified Customer | Posted 02/04/2022
    Blue: DAPI; Green: SARS-CoV-2 membrane protein (NB100-56569); Magenta: SARS-CoV-2 nuclecapsid protein. Vero cells infected with SARS-CoV-2.
    Sars Membrane Protein Antibody - BSA Free NB100-56569
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs for Sars Membrane Protein Antibody - BSA Free

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  • Q: Has it been tested whether the two antobodies anti-M protein from SARS-CoV cross-react with that of SARS-CoV-2? The references are 56569SS and NBP1-28852.

    A: Sars Membrane Protein Antibody NB100-56569 expected cross reactivity with SARS-CoV-2 based on immunogen sequence homology: 93%. Sars Membrane Protein Antibody (2H2C4)
    NBP1-28852 expected cross reactivity with SARS-CoV-2 based on immunogen sequence homology: 88%

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