SARS Spike Protein Antibody - BSA Free
Novus Biologicals | Catalog # NB100-56578
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Scientific Data Images for SARS Spike Protein Antibody - BSA Free
Simple Western Detection of Recombinant SARS-CoV-2 Spike S2 Protein Using SARS Spike Protein Antibody
Simple Western lane view shows recombinant SARS-CoV-2 Spike S2 Protein, loaded at 50 ng/mL. A specific band was detected for SARS-CoV-2 Spike S2 Protein at approximately 58 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Spike Protein Polyclonal Antibody (Catalog # NB100-56578) followed by incubation with HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Detection of SARS-CoV-2 Spike in 293T Cell Lysate Using SARS Spike Protein Antibody
SARS-CoV-2 spike overexpressed 293T cell lysate. Antibody dilution: 1:3000. Two bands for full length spike and cleaved S2 are shown, respectively. Western blot image submitted by a verified customer review.
Western Blot Detection of SARS-CoV-1 and SARS CoV-2 Spike Protein Using SARS Spike Protein Antibody
Western blot shows SARS-CoV-1 Spike (Catalog # 10581-CV), SARS CoV-2 Spike (Catalog # 10586-CV), SArS-CoV-2 Spike S2 Subunit (Catalog # 10584-CV). Membrane was probed with 0.5 ug/mL of SARS Spike Protein Antibody (Catalog # NB100-56578) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.
Detection of SARS Spike Protein in Transfected and Untransfected Mouse Melanoma Cell Lysate by Western Blot
Western blot analysis of SARS Spike Protein in (A) transfected and (B) untransfected mouse melanoma cell lysate.
Western Blot Detection of Recombinant SARS-CoV-2 Spike S2 Subunit Using SARS Spike Protein Antibody
Western blot shows recombinant protein SARS-CoV-2 Spike S2 Subunit. Membrane was probed with 0.5 ug/mL of Rabbit Anti-SARS Spike Protein Polyclonal Antibody (Catalog # NB100-56578) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.
Simple Western Detection of SARS Spike Protein in SARS-CoV-2 Lysate
Simple Western lane view shows SARS-CoV-2 lysate, loaded at 1:20 dilution. SARS-CoV-2 Spike Protein was detected using 6.25 ug/mL of Rabbit Anti-SARS-CoV Spike Protein Polyclonal Antibody (Catalog # NB100-56578) followed by incubation with HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. SARS-CoV-2 lysate courtesy of University of Maryland.Applications for SARS Spike Protein Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Simple Western
Western Blot
Reviewed Applications
Read 1 review rated 5 using NB100-56578 in the following applications:
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Background: SARS Spike Protein
Alternate Names
Additional SARS Spike Protein Products
Product Documents for SARS Spike Protein Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for SARS Spike Protein Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SARS Spike Protein Antibody - BSA Free
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Customer Images
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Application: Western BlotSample Tested: SARS-CoV-2 spike transfected 293T cell lysateSpecies: HumanVerified Customer | Posted 10/02/2020SARS-CoV-2 spike overexpressed 293T cell lysate Antibody dilution: 1:3000 and two bands for full length spike and cleaved S2 were shown, respectively.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for SARS Spike Protein Antibody - BSA Free
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Q: Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
A: NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
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Q: Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
A: NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
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Q: I am looking for a primary antibody to sars spike protein to the new COVID-19.
A: NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
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Q: The spec sheet says that NB100-56578 species cross reactivity 100% wonder what that means…
A: It means we have blasted homology between our immunogens and COVID19, and this one is 100% homologous. However, we do not have COVID19 samples to validate with, so it is predicted to work on COVID19.
-
Q: Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
A: NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
-
Q: Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
A: NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
-
Q: I am looking for a primary antibody to sars spike protein to the new COVID-19.
A: NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
-
Q: The spec sheet says that NB100-56578 species cross reactivity 100% wonder what that means…
A: It means we have blasted homology between our immunogens and COVID19, and this one is 100% homologous. However, we do not have COVID19 samples to validate with, so it is predicted to work on COVID19.
-
Q: Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
A: NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
-
Q: Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
A: NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
-
Q: I am looking for a primary antibody to sars spike protein to the new COVID-19.
A: NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
-
Q: The spec sheet says that NB100-56578 species cross reactivity 100% wonder what that means…
A: It means we have blasted homology between our immunogens and COVID19, and this one is 100% homologous. However, we do not have COVID19 samples to validate with, so it is predicted to work on COVID19.
-
Q: Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
A: NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
-
Q: Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
A: NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
-
Q: I am looking for a primary antibody to sars spike protein to the new COVID-19.
A: NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
-
Q: The spec sheet says that NB100-56578 species cross reactivity 100% wonder what that means…
A: It means we have blasted homology between our immunogens and COVID19, and this one is 100% homologous. However, we do not have COVID19 samples to validate with, so it is predicted to work on COVID19.