SATB1 Antibody

Novus Biologicals | Catalog # NBP2-15107

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (91%), Rat (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SATB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SATB1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SATB1 Antibody

Western Blot: SATB1 Antibody [NBP2-15107]

Western Blot: SATB1 Antibody [NBP2-15107]

Western Blot: SATB1 Antibody [NBP2-15107] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107] - Mouse lymph node. SATB1 stained by SATB1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate
Chromatin Immunoprecipitation: SATB1 Antibody [NBP2-15107] - Cross-linked ChIP was performed with Jurkat chromatin extract and 5 ug of either control rabbit IgG or anti-SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.ChIP experiment and primer designs are based on Nucleic Acids Res. 2011 Jun;39(11):4640-52.
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107] - Mouse thymus gland. SATB1 stained by SATB1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citra
Immunoprecipitation: SATB1 Antibody [NBP2-15107]

Immunoprecipitation: SATB1 Antibody [NBP2-15107]

Immunoprecipitation: SATB1 Antibody [NBP2-15107] - 293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 ug of SATB1 antibody 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
SATB1 Antibody

Western Blot: SATB1 Antibody [NBP2-15107] -

Western Blot: SATB1 Antibody [NBP2-15107] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (NBP2-15107) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SATB1 Antibody

Immunocytochemistry/ Immunofluorescence: SATB1 Antibody [NBP2-15107] -

Immunocytochemistry/ Immunofluorescence: SATB1 Antibody [NBP2-15107] - SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SATB1 stained by SATB1 antibody [C2C3], C-term (NBP2-15107) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for SATB1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000
Application Notes
ChIP assay-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SATB1

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

SATB Homeobox 1

Alternate Names

DNA-binding protein SATB1, SATB homeobox 1, special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's), Special AT-rich sequence-binding protein 1

Gene Symbol

SATB1

UniProt

Q01826

Additional SATB1 Products

Product Documents for SATB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SATB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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