SATB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15108

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Chicken (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SATB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SATB1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SATB1 Antibody - BSA Free

Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108] - Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody diluted by 1:500
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108] - SATB1 antibody detects SATB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. SATB1 antibody dilution: 1:500.
Chromatin Immunoprecipitation: SATB1 Antibody [NBP2-15108] - Analysis using SATB1 and SATB2 antibodies on Bcl2 promoter. Image submitted by a verified customer review.
Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108] - Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody diluted by 1:500
Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108]

Western Blot: SATB1 Antibody [NBP2-15108] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108] - Paraffin-embedded TOV-21G xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108]

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108] - SATB1 antibody detects SATB1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SATB1 antibody dilution: 1:500.

Applications for SATB1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP2-15108 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SATB1

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

SATB Homeobox 1

Alternate Names

DNA-binding protein SATB1, SATB homeobox 1, special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's), Special AT-rich sequence-binding protein 1

Gene Symbol

SATB1

UniProt

Q01826

Additional SATB1 Products

Product Documents for SATB1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SATB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for SATB1 Antibody - BSA Free

Customer Reviews for SATB1 Antibody - BSA Free (1)

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Customer Images

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  • Name: Ranveer Singh
    Application: Chromatin Immunoprecipitation
    Sample Tested: Cross-linked chromatin from HEK 293T cells
    Species: Human
    Verified Customer | Posted 06/18/2014
    ChIP with SATB1 and SATB2 antibodies on Bcl2 promoter
    SATB1 Antibody - BSA Free NBP2-15108

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Protocols

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FAQs

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