SATB2 Antibody (CL0321) - BSA Free

Novus Biologicals | Catalog # NBP2-52915

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0321

Format

BSA Free
View all SATB2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9UPW6, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SATB2 Antibody (CL0321) - BSA Free

Analysis in human rectum and liver tissues using AMAb90680 antibody. Corresponding SATB2 RNA-seq data are presented for the same tissues.

Analysis in human rectum and liver tissues using AMAb90680 antibody. Corresponding SATB2 RNA-seq data are presented for the same tissues.
Western Blot: SATB2 Antibody (CL0321) [NBP2-52915]

Western Blot: SATB2 Antibody (CL0321) [NBP2-52915]

Western Blot: SATB2 Antibody (CL0321) [NBP2-52915] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: SATB2 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry-Paraffin: SATB2 Antibody (CL0321) [NBP2-52915]

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0321) [NBP2-52915]

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0321) [NBP2-52915] - Staining of rat hippocampal formation shows strong nuclear immunoreactivity in CA1 pyramidal cell layer.
Western Blot: SATB2 Antibody (CL0321) [NBP2-52915]

Western Blot: SATB2 Antibody (CL0321) [NBP2-52915]

Western Blot: SATB2 Antibody (CL0321) [NBP2-52915] - Lane 1: Marker [kDa], Lane 2: Human cell line HEL

Staining of human rectum shows strong nuclear positivity in glandular cells.

Staining of human rectum shows strong nuclear positivity in glandular cells.

Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Staining of human colorectal cancer shows moderate to strong nuclear positivity in tumor cells.

Staining of human colorectal cancer shows moderate to strong nuclear positivity in tumor cells.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of rat hippocampus shows no nuclear positivity in neurons in the dentate gyrus as expected.

Staining of rat hippocampus shows no nuclear positivity in neurons in the dentate gyrus as expected.

Applications for SATB2 Antibody (CL0321) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SATB2

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Alternate Names

DNA-binding protein SATB2, FLJ32076, KIAA1034FLJ21474, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, Special AT-rich sequence-binding protein 2

Gene Symbol

SATB2

Additional SATB2 Products

Product Documents for SATB2 Antibody (CL0321) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SATB2 Antibody (CL0321) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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