SATB2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83619

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SATB2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VSQAVFARVAFNRTQGLLSEILRKEEDPRTASQSLLVNLRAMQNFLNLPEVERDRIYQDERERSMNPNVSMVSSASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDEIQQEMKRAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SATB2 Antibody - BSA Free

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Analysis in human colon and liver tissues. Corresponding SATB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human cerebral cortex, colon, liver and rectum using Anti-SATB2 antibody NBP1-83619 (A) shows similar protein distribution across tissues to independent antibody NBP1-92365 (B).
Western Blot: SATB2 Antibody [NBP1-83619]

Western Blot: SATB2 Antibody [NBP1-83619]

Western Blot: SATB2 Antibody [NBP1-83619] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human liver shows no positivity as expected.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human colon shows strong nuclear positivity in glandular cells.
Simple Western: SATB2 Antibody [NBP1-83619]

Simple Western: SATB2 Antibody [NBP1-83619]

Simple Western: SATB2 Antibody [NBP1-83619] - Simple Western lane view shows a specific band for SATB2 in 0.1 mg/ml of THP1 (left), HDLM2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: SATB2 Antibody [NBP1-83619]

Simple Western: SATB2 Antibody [NBP1-83619]

Simple Western: SATB2 Antibody [NBP1-83619] - Electropherogram image(s) of corresponding Simple Western lane view. SATB2 antibody was used at 1:50 dilution on THP1 and HDLM2 lysate(s).

Applications for SATB2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:50

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in THP1, HDLM2, separated by Size, antibody dilution of 1:50, apparent MW was 112 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SATB2

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Alternate Names

DNA-binding protein SATB2, FLJ32076, KIAA1034FLJ21474, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, Special AT-rich sequence-binding protein 2

Gene Symbol

SATB2

Additional SATB2 Products

Product Documents for SATB2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SATB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SATB2 Antibody - BSA Free

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Protocols

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