SDNSF/MCFD2 Antibody (3A5-G4) - Azide and BSA Free
Novus Biologicals | Catalog # H00090411-M01
![Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]](https://resources.rndsystems.com/images/products/SDNSF-MCFD2-Antibody-3A5-G4-Western-Blot-H00090411-M01-img0012.jpg "Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A5-G4
Format
Azide and BSA Free
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Product Specifications
Immunogen
MCFD2 (AAH40357, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMRSLLRTPFLCGLLWAFCAPGARAEEPAASFSQPGSMGLDKNTVHDQEHIMEHLEGVINKPEAEMSPQELQLHYFKMHDYDGNNLLDGLELSTAITHVHKEEGSEQAPLMSEDELINIIDGVLRDDDKNNDGYIDYAEFAKSLQ
Reactivity Notes
Human. Other species not tested.
Specificity
MCFD2 - multiple coagulation factor deficiency 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for SDNSF/MCFD2 Antibody (3A5-G4) - Azide and BSA Free
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 expression in transfected 293T cell line by MCFD2 monoclonal antibody (M01), clone 3A5-G4.Lane 1: MCFD2 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]](https://resources.rndsystems.com/images/products/SDNSF-MCFD2-Antibody-3A5-G4-Immunohistochemistry-Paraffin-H00090411-M01-img0008.jpg "Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]")
Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]
Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of monoclonal antibody to MCFD2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.![Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]](https://resources.rndsystems.com/images/products/SDNSF-MCFD2-Antibody-3A5-G4-Western-Blot-H00090411-M01-img0009.jpg "Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]")
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 over-expressed 293 cell line, cotransfected with MCFD2 Validated Chimera RNAi ( Cat # H00090411-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCFD2 monoclonal antibody (M01), clone 3A5-G4 (Cat # H00090411-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Detection limit for recombinant GST tagged MCFD2 is 0.1 ng/ml as a capture antibody.
Applications for SDNSF/MCFD2 Antibody (3A5-G4) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein for Western Blot. It has also been used for immunohistochemistry (paraffin), ELISA and RNAi validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SDNSF/MCFD2
Long Name
Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor
Alternate Names
MCFD2
Entrez Gene IDs
90411 (Human)
Gene Symbol
MCFD2
OMIM
227 (Human)
UniProt
Additional SDNSF/MCFD2 Products
Product Documents for SDNSF/MCFD2 Antibody (3A5-G4) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SDNSF/MCFD2 Antibody (3A5-G4) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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